Feasibility and safety of synchrotron-based X-ray phase contrast imaging as a technique complementary to histopathology analysis

被引：6

作者：

Li, Kan Yan Chloe ^{[1
]}

Dejea, Hector ^{[2
,3
,4
,5
]}

De Winne, Koen ^{[6
]}

Bonnin, Anne ^{[2
]}

D'Onofrio, Valentino ^{[7
]}

Cox, Janneke A. ^{[7
,8
]}

Garcia-Canadilla, Patricia ^{[9
,10
,11
]}

Lammens, Martin ^{[6
]}

Cook, Andrew C. ^{[1
]}

Bijnens, Bart

Dendooven, Amelie ^{[6
,7
]}

机构：

[1] UCL, Inst Cardiovasc Sci, London, England

[2] Paul Scherrer Inst, Swiss Light Source, Villigen, Switzerland

[3] Swiss Fed Inst Technol, Zurich, Switzerland

[4] Lund Univ, Dept Biomed Engn, Lund, Sweden

[5] MAX IV Lab, Lund, Sweden

[6] Antwerp Univ Hosp, Univ Antwerp, Dept Pathol, Antwerp, Belgium

[7] Hasselt Univ, Fac Med & Life Sci, Hasselt, Belgium

[8] Jessa Hosp, Dept Infect Dis & Immun, Hasselt, Belgium

[9] St Joan Deu Res Inst IRSJD, Interdisciplinary Cardiovasc Res Grp, Barcelona, Spain

[10] Univ Barcelona, Hosp Sant Joan Deu, BCNatal Barcelona Ctr Maternal Fetal & Neonatal Me, Barcelona, Spain

[11] Univ Barcelona, Hosp Clin, Barcelona, Spain

来源：

HISTOCHEMISTRY AND CELL BIOLOGY | 2023年 / 160卷 / 05期

基金：

欧盟地平线“2020”;

关键词：

X-ray phase contrast imaging; Pathology; Needle biopsies; CANCER SPECIMENS; TISSUE SAMPLES; CT; TOMOGRAPHY; EXTRACTION; MICROSCOPY; HISTOLOGY;

D O I：

10.1007/s00418-023-02220-6

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

X-ray phase contrast imaging (X-PCI) is a powerful technique for high-resolution, three-dimensional imaging of soft tissue samples in a non-destructive manner. In this technical report, we assess the quality of standard histopathological techniques performed on formalin-fixed, paraffin-embedded (FFPE) human tissue samples that have been irradiated with different doses of X-rays in the context of an X-PCI experiment. The data from this study demonstrate that routine histochemical and immunohistochemical staining quality as well as DNA and RNA analyses are not affected by previous X-PCI on human FFPE samples. From these data we conclude it is feasible and acceptable to perform X-PCI on FFPE human biopsies.

引用

页码：377 / 389

页数：13

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