Deciphering Salt Tolerance in Cotton: Unveiling Insights from Genetic Diversity Seedling Stage Growth Parameters

Salinity stress is a major threat to cotton growth and productivity worldwide. This study evaluated a diverse collection of fifty cotton genotypes at critical seedling stage using analysis of variance and principal component analysis. The seedlings were subjected to 10dSm-1 and 17dSm-1 salt stress levels starting from the 10th day after sowing until the 42nd days. Data was collected on morphological (root length, shoot length, fresh and dry weights of root and shoot, fresh and dry weight of whole plant) and biochemical (K+, Na+, K+/Na+) parameters. Significant variations were observed among the cotton genotypes for the evaluated traits under control and salt stress conditions. The results revealed that salinity stress had detrimental effects on various growth parameters, including root length, shoot length, fresh root weight, fresh shoot weight, dry shoot weight, dry root weight, and whole plant dry weight. Additionally, sodium levels exhibited a substantial increase under NaCl-induced stress, whereas potassium levels experienced reductions in the leaves. Based on multivariate analysis, the genotypes NIAB545, FH- Kehkshan, FH-490, and GH-Mubarak displayed notable salt tolerance likely due to mechanisms such as ion exclusion, osmotic adjustment and tissue tolerance at both 10 dSm-1 and 17 dSm-1 levels. Conversely, the genotypes FH-303, FH-532, FH-542, and SB-149 were deemed salt -sensitive. These findings underscore the challenges associated with selection for salt tolerance in cotton due to the potential masking effects of environmental factors on genetic expression. This study discovers potential salt -tolerant cotton genotypes for use in breeding programs to increase yields under saline stress. However, all genotype -environment interactions must be explored. Overall, these findings provide useful information about salt stress responses in cotton seedlings