The bone-protective benefits of amino-conjugated calcium in an ovariectomized (OVX) rat model

被引：4

作者：

Ali, Maqsood ^{[1
,2
]}

Lee, Youri ^{[1
,2
]}

Ha, Bin ^{[3
,5
]}

Jung, Jaeeun ^{[4
]}

Lee, Byoung-Ryol ^{[1
,6
]}

Kim, Dae-soo ^{[4
]}

Lee, Mi-Young ^{[3
,5
]}

Kim, Yong-Sik ^{[1
,2
,7
]}

机构：

[1] Soonchunhyang Univ, Coll Med, Dept Microbiol, Cheonan 31151, Chungnam, South Korea

[2] Soonchunhyang Univ, Inst Tissue Regenerat, Coll Med, Cheonan 31151, Chungnam, South Korea

[3] Soonchunhyang Univ, Coll Med Sci, Dept Med Sci, Asan 31538, Chungnam, South Korea

[4] Korea Res Inst Biosci & Biotechnol, Daejeon 34141, South Korea

[5] Soonchunhyang Univ, Coll Med Sci, Dept Med Biotechnol, Asan 31538, Chungnam, South Korea

[6] BTN Co Ltd, 407ho, Entrepreneurship Hall, 22 Soonchunhyang ro, Asan 31538, Chungnam, South Korea

[7] Soonchunhyang Univ, Coll Med, Dept Microbiol, Soonchunhyang 6 gil 31, Cheonan 31151, Chungnam, South Korea

来源：

LIFE SCIENCES | 2023年 / 328卷

关键词：

Amino-calcium; Bone protection; Ovariectomized; Osteoporosis; RNA-sequencing; POSTMENOPAUSAL WOMEN; MINERAL DENSITY; OSTEOBLAST DIFFERENTIATION; OSTEOPOROSIS PREVENTION; SIGNALING PATHWAYS; PROXIMAL FEMUR; TURNOVER; ACID; ASSOCIATION; ESTROGEN;

D O I：

10.1016/j.lfs.2023.121927

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Low bone density, fragility, and microarchitectural disintegration are the symptoms of osteoporosis. An imbal-ance between bone growth and resorption can lead to osteoporosis. This study evaluated the effects of amino -calcium (AC) on bone protection in ovariectomized control group (NC) rats. Amino-calcium (AC) was charac-terized using Fourier-transform infrared spectroscopy (FT-IR), energy-dispersive X-ray spectroscopy (EDS), and nuclear magnetic resonance spectroscopy analyses (NMR). After determining the biocompatibility of amino -calcium (AC) with MC3T3-E1 cells, alkaline phosphatase staining revealed significant changes on day 7. Three of the four groups underwent ovariectomy, whereas one group received a placebo. On micro-computed to-mography, in vivo, data showed increased bone volume fraction in the femoral head and shaft areas in the amino -calcium (AC) group. Hematoxylin and eosin staining showed a bone mass and architectural protection in the amino-calcium (AC) group compared with the calcium carbonate and OVX control group. RNA sequencing analysis revealed high expression of osteogenesis-related genes in MC3T3-E1 cells. RNA sequencing revealed a significant fold change in the expression of integrin-binding sialoprotein (IBSP), bone gamma-carboxyglutamate proteins 1 and 2(BGLAP1 and BGLAP2), and periostin (POSTN). The study concluded that supplementing the OVX rats with calcium enhanced bone protection.

引用

页数：11

共 89 条

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