Effect of HHCB and Cd on phytotoxicity, accumulation, subcellular distribution and stereoselectivity of chiral HHCB in soil-plant systems

The toxicity of HHCB in the growth and development of plants is well known, but its uptake, subcellular dis-tribution, and stereoselectivity, especially in a co-contamination environment, is not fully understood. Therefore, a pot experiment was performed to research the physiochemical response, and the fate of HHCB in pakchoi when the Cd co-existed in soil. The Chl contents were significantly lower, and the oxidative stress was aggravated under the co-exposure of HHCB and Cd. The accumulations of HHCB in roots were inhibited, and those in leaves were elevated. The transfer factors of HHCB in HHCB-Cd treatment increased. The subcellular distributions were analyzed in the cell walls, cell organelles, and cell soluble constituents of roots and leaves. In roots, the distri-bution proportion of HHCB followed cell organelle > cell wall > cell soluble constituent. In leaves, the distri-bution proportion of HHCB was different from that in roots. And the co-existing Cd made the distribution proportion of HHCB change. In the absence of Cd, the (4R,7S)-HHCB and (4R,7R)-HHCB were preferentially enriched in roots and leaves, and the stereoselectivity of chiral HHCB was more significant in roots than leaves. The co-existing Cd reduced the stereoselectivity of HHCB in plants. Our findings suggested that the fate of HHCB was affected by the co-existing Cd, so the risk of HHCB in the complicated environment should be paid more attention.