Biologically Synthesized Silver Nanoparticles Efficiently Control Plant Pathogenic Bacteria-Erwinia carotovora and Ralstonia solanacearum

Biogenic nanoparticles are considered effective alternatives to chemical pesticides for the management of pathogenic plant diseases. This study was focused on the synthesis of stable silver nanoparticles (AgNPs) to control challenging plant pathogenic bacteria in vitro and in planta. We synthesized AgNPs by reacting different proportions of silver nitrate and aqueous extract of Hedera nepalensis. The physicochemical properties of the synthesized AgNPs were determined by using various physical techniques. The TEM analysis revealed the AgNPs less than 50 nm in size and spherical shaped. For antibacterial assays, different concentrations (1000-15.62 & mu;g/mL, 2-fold dilutions) of the extract-free AgNPs (Ef-AgNPs) or extract-mixed AgNPs (Em-AgNPs), and fruit extracts (FE) were used against plant pathogenic bacteria Erwinia carotovora subsp. carotovora, Erwinia carotovora subsp. atroseptica, and Ralstonia solanacearum. In the in vitro assays, we found significant inhibition of both bacterial species in response to maximum concentrations of AgNPs. Overall, Ef-AgNPs exhibited a higher percent inhibition of bacterial pathogens. In potato tubers assay, complete inhibition of Erwinia carotovora was observed, except for the lowest AgNPs concentration of 15.62 & mu;g/mL. Similarly, exposure of tomato plants to Ralstonia solanacearum suspensions (OD600 = 0.2) in the soil-drenching experiment and post-exposure treatment with 1000 & mu;g/mL and 125 & mu;g/mL of AgNPs resulted in disease inhibition. This study provides the basis that biogenic nanoparticles prepared from Hedera nepalensis are one of the best substitutes to synthetic pesticide, having displayed better results to control the growth of phytopathogenic microbes. However, field studies need to be conducted in a controlled environment to scale up the current work and find out the efficacy of nanoparticles on a larger scale.