Fabricating ZSM-5 zeolite/ polycaprolactone nano-fibers co-loaded with dexamethasone and ascorbic acid: Potential application in osteogenic differentiation of human adipose-derived stem cells

被引：15

作者：

Mofarrah, Mohsen ^{[1
,2
]}

Jafari-Gharabaghlou, Davoud ^{[3
]}

Dadashpour, Mehdi ^{[4
,5
]}

Zarghami, Nosratollah ^{[2
,3
,6
,7
]}

机构：

[1] Tabriz Univ Med Sci, Fac Adv Med Sci, Dept Med Biotechnol, Tabriz, Iran

[2] Tabriz Univ Med Sci, Stem Cell Res Ctr, Tabriz, Iran

[3] Univ Med Sci, Fac Med, Dept Clin Biochem & Lab Med, Tabriz, Iran

[4] Semnan Univ Med Sci, Canc Res Ctr, Semnan, Iran

[5] Semnan Univ Med Sci, Fac Med, Dept Biotechnol, Semnan, Iran

[6] Istanbul Aydin Univ, Fac Med, Dept Med Biochem, Istanbul, Turkiye

[7] Tabriz Univ Med Sci, Fac Med, Clin Biochem & Lab Med, Tabriz, Iran

来源：

JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY | 2023年 / 79卷

关键词：

Dexamethasone; Ascorbic acid; ZSM-5; zeolite; Osteoblastic differentiation; Electrospun nanofibers; BONE; SCAFFOLDS;

D O I：

10.1016/j.jddst.2022.103999

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

In this study, the efficient ZSM-5 zeolite/polycaprolactone-polyethylene glycol nanofibers (PCL-PEG NFs) were developed for the combined sustained release of dexamethasone (DEX), and ascorbic acid (ASC) to enhance the osteoblastic differentiation of human adipose-derived stem cells (hADSCs). The fabricated NFs were evaluated by BET/BJH, FTIR, and FE-SEM analyses. This composite DEX/ASC@ZSM-5/PCL-PEG electrospun NFs revealed that the initial burst drug release was rather reduced by loading the drug into the ZSM-5 zeolites, resulting in a sustained and prolonged drug release from NFs. The results of FE-SEM, MTT and PicoGreen assays, qPCR, and alkaline phosphatase (ALP) assays confirmed hADSCs cultured on the DEX/ASC@ZSM-5/PCL-PEG NFs had su-perior cell adhesion, metabolic activity, and proliferation percentage compared to other forms of NFs after 14 and 28 days of incubation. In the meantime, DEX and ASC displayed high osteogenic differentiation capability when they impacted on hADSCs. These results indicated that ZSM-5/NFs could be used to develop a novel platform for bone tissue regeneration and engineering by facilitating of the sustained/controlled release of therapeutic compounds.

引用

页数：10