Parkinson's disease (PD) is characterized by substantial phenotypic heterogeneity that limits the disease prognosis and patient's counseling, and complicates the design of further clinical trials. There is an unmet need for the development and validation of biomarkers for the prediction of the disease course. In this study, we utilized flow cytometry and in vitro approaches on peripheral blood cells and isolated peripheral blood mononuclear cell (PBMC)-derived macrophages to characterize specific innate immune populations in PD patients versus healthy donors. We found a significantly lower percentage of B lymphocytes and monocyte populations in PD patients. Monocytes in PD patients were characterized by a higher CD40 expression and on-surface expression of the type I membrane glycoprotein sortilin, which showed a trend of negative correlation with the age of the patients. These results were further investigated in vitro on PBMC-derived macrophages, which, in PD patients, showed higher sortilin expression levels compared to cells from healthy donors. The treatment of PD-derived macrophages with oxLDL led to higher foam cell formation compared to healthy donors. In conclusion, our results support the hypothesis that surface sortilin expression levels on human peripheral monocytes may potentially be utilized as a marker of Parkinson's disease and may segregate the sporadic versus the genetically induced forms of the disease.
机构:
Univ Cattolica Sacro Cuore, Fac Med & Chirurg, Dipartimento Neurosci, Sez Neurol, I-00168 Rome, Italy
Fdn Policlin Univ Agostino Gemelli IRCCS, Neurol, I-00168 Rome, ItalyUniv Cattolica Sacro Cuore, Fac Med & Chirurg, Dipartimento Neurosci, Sez Neurol, I-00168 Rome, Italy
Calabresi, Paolo
Mechelli, Alessandro
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Magna Graecia Univ Catanzaro, Ist Neurol, Dipartimento Sci Med & Chirurg, Catanzaro, ItalyUniv Cattolica Sacro Cuore, Fac Med & Chirurg, Dipartimento Neurosci, Sez Neurol, I-00168 Rome, Italy
Mechelli, Alessandro
Natale, Giuseppina
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Univ Cattolica Sacro Cuore, Fac Med & Chirurg, Dipartimento Neurosci, Sez Neurol, I-00168 Rome, ItalyUniv Cattolica Sacro Cuore, Fac Med & Chirurg, Dipartimento Neurosci, Sez Neurol, I-00168 Rome, Italy
Natale, Giuseppina
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Volpicelli-Daley, Laura
Di Lazzaro, Giulia
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Fdn Policlin Univ Agostino Gemelli IRCCS, Neurol, I-00168 Rome, ItalyUniv Cattolica Sacro Cuore, Fac Med & Chirurg, Dipartimento Neurosci, Sez Neurol, I-00168 Rome, Italy
Di Lazzaro, Giulia
Ghiglieri, Veronica
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Fdn Policlin Univ Agostino Gemelli IRCCS, Neurol, I-00168 Rome, Italy
Univ Telematica San Raffaele, I-00166 Rome, ItalyUniv Cattolica Sacro Cuore, Fac Med & Chirurg, Dipartimento Neurosci, Sez Neurol, I-00168 Rome, Italy
机构:
Univ Cattolica Sacro Cuore, Fac Med & Chirurg, Dipartimento Neurosci, Sez Neurol, I-00168 Rome, Italy
Fdn Policlin Univ Agostino Gemelli IRCCS, Neurol, I-00168 Rome, ItalyUniv Cattolica Sacro Cuore, Fac Med & Chirurg, Dipartimento Neurosci, Sez Neurol, I-00168 Rome, Italy
Calabresi, Paolo
Mechelli, Alessandro
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h-index: 0
机构:
Magna Graecia Univ Catanzaro, Ist Neurol, Dipartimento Sci Med & Chirurg, Catanzaro, ItalyUniv Cattolica Sacro Cuore, Fac Med & Chirurg, Dipartimento Neurosci, Sez Neurol, I-00168 Rome, Italy
Mechelli, Alessandro
Natale, Giuseppina
论文数: 0引用数: 0
h-index: 0
机构:
Univ Cattolica Sacro Cuore, Fac Med & Chirurg, Dipartimento Neurosci, Sez Neurol, I-00168 Rome, ItalyUniv Cattolica Sacro Cuore, Fac Med & Chirurg, Dipartimento Neurosci, Sez Neurol, I-00168 Rome, Italy
Natale, Giuseppina
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机构:
Volpicelli-Daley, Laura
Di Lazzaro, Giulia
论文数: 0引用数: 0
h-index: 0
机构:
Fdn Policlin Univ Agostino Gemelli IRCCS, Neurol, I-00168 Rome, ItalyUniv Cattolica Sacro Cuore, Fac Med & Chirurg, Dipartimento Neurosci, Sez Neurol, I-00168 Rome, Italy
Di Lazzaro, Giulia
Ghiglieri, Veronica
论文数: 0引用数: 0
h-index: 0
机构:
Fdn Policlin Univ Agostino Gemelli IRCCS, Neurol, I-00168 Rome, Italy
Univ Telematica San Raffaele, I-00166 Rome, ItalyUniv Cattolica Sacro Cuore, Fac Med & Chirurg, Dipartimento Neurosci, Sez Neurol, I-00168 Rome, Italy