Mixotrophic culture of Chaetoceros sp. and the synergistic carbon and energy metabolism

被引：6

作者：

Shan, Shengzhou ^{[1
]}

Wang, Shanshan ^{[2
]}

Yan, Xi ^{[1
]}

Chen, Kang ^{[1
,3
]}

Liang, Li ^{[4
]}

Li, Xiaohui ^{[1
]}

Zhou, Chengxu ^{[1
]}

Yan, Xiaojun ^{[5
]}

Ruan, Roger ^{[6
,7
]}

Cheng, Pengfei ^{[1
,6
,7
]}

机构：

[1] Ningbo Univ, Coll Food & Pharmaceut Sci, Ningbo 315211, Zhejiang, Peoples R China

[2] Ningbo Univ, Affiliated Hosp 1, Ningbo 315211, Zhejiang, Peoples R China

[3] Univ Turku, Dept Biochem, Food Chem & Food Dev, FI-20014 Turku, Finland

[4] Jiangnan Univ, State Key Lab Food Sci & Resources, Wuxi 214122, Peoples R China

[5] Ningbo Univ, Key Lab Marine Biotechnol Zhejiang Prov, Ningbo 315211, Zhejiang, Peoples R China

[6] Univ Minnesota Twin Cities, Ctr Biorefining, St Paul, MN 55108 USA

[7] Univ Minnesota Twin Cities, Dept Bioprod & Biosyst Engn, St Paul, MN 55108 USA

来源：

BIORESOURCE TECHNOLOGY | 2023年 / 390卷

基金：

国家重点研发计划; 中国国家自然科学基金;

关键词：

Chaetoceros sp; Mixotrophic culture; Carbon metabolism; Energy metabolism; Synergistic mechanism; MICROALGAE; CULTIVATION; BIOMASS;

D O I：

10.1016/j.biortech.2023.129912

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

This research studied the metabolic mechanism of the mixotrophic Chaetoceros sp. The results showed this alga had the highest cell density and growth rate of 47.72 x 10(5) cells mL(-1) and 0.41 d(-1), respectively, with a maximum dry weight of 2.90 g/L, when compared to photoautotrophic and photoheterotrophic modes. Compared to photoheterotrophy, transcriptomics results showed the Rubisco, PGK, and GAPDH related genes were separately up-regulated by 1.03, 2.36, and 1.36 times in CBB cycle in mixotrophic mode, suggesting intermediate metabolites of EMP and PPP can enter the chloroplast via transporter proteins, or membrane permeation, and feedback inhibition regulates the reduction of multiple reactions in CBB cycle. Chaetoceros sp. achieves high biomass by utilizing ATP and carbon structures from EMP and PPP pathways, and the addition of NaHCO3 leads to an up-regulation of CBB cycle for the mixotrophic alga, resulting in higher biomass compared to the photoheterotrophic mode.

引用

页数：11

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