Comparison of Long-Read Methods for Sequencing and Assembly of Lepidopteran Pest Genomes

被引:11
作者
Zhang, Tong [1 ,2 ]
Xing, Weiqing [1 ,2 ]
Wang, Aoming [1 ,2 ]
Zhang, Na [2 ]
Jia, Ling [1 ,2 ]
Ma, Sanyuan [1 ,2 ]
Xia, Qingyou [1 ,2 ]
机构
[1] Southwest Univ, Biol Sci Res Ctr, State Key Lab Silkworm Genome Biol, Chongqing 400715, Peoples R China
[2] Chongqing Engn & Technol Res Ctr Novel Silk Mat, Chongqing Key Lab Sericulture Sci, Chongqing 400715, Peoples R China
基金
中国国家自然科学基金;
关键词
biological control; de novo assembly; long-read sequencing; benchmarking; lepidopteran pest; assembly evaluation; PROVIDES; FUTURE; SYSTEM;
D O I
10.3390/ijms24010649
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Lepidopteran species are mostly pests, causing serious annual economic losses. High-quality genome sequencing and assembly uncover the genetic foundation of pest occurrence and provide guidance for pest control measures. Long-read sequencing technology and assembly algorithm advances have improved the ability to timeously produce high-quality genomes. Lepidoptera includes a wide variety of insects with high genetic diversity and heterozygosity. Therefore, the selection of an appropriate sequencing and assembly strategy to obtain high-quality genomic information is urgently needed. This research used silkworm as a model to test genome sequencing and assembly through high-coverage datasets by de novo assemblies. We report the first nearly complete telomere-to-telomere reference genome of silkworm Bombyx mori (P50T strain) produced by Pacific Biosciences (PacBio) HiFi sequencing, and highly contiguous and complete genome assemblies of two other silkworm strains by Oxford Nanopore Technologies (ONT) or PacBio continuous long-reads (CLR) that were unrepresented in the public database. Assembly quality was evaluated by use of BUSCO, Inspector, and EagleC. It is necessary to choose an appropriate assembler for draft genome construction, especially for low-depth datasets. For PacBio CLR and ONT sequencing, NextDenovo is superior. For PacBio HiFi sequencing, hifiasm is better. Quality assessment is essential for genome assembly and can provide better and more accurate results. For chromosome-level high-quality genome construction, we recommend using 3D-DNA with EagleC evaluation. Our study references how to obtain and evaluate high-quality genome assemblies, and is a resource for biological control, comparative genomics, and evolutionary studies of Lepidopteran pests and related species.
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页数:17
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