The angiogenesis-modulating effects of coumarin-derivatives

被引:2
作者
Huang, Han-Ting [1 ]
Huang, Ching-Yuan [1 ]
Lee, Chih-Jou [1 ]
Sun, Bo-Jie [1 ]
Jhang, Zong-Wei [4 ]
Wen, Chi -Chung [2 ]
Wang, Yun-Hsin [1 ]
Li, Tao -Sheng [3 ]
Chern, Ching-Yuh [4 ,5 ]
Chen, Yau-Hung [1 ,5 ]
机构
[1] Tamkang Univ, Dept Chem, Tamsui, Taiwan
[2] Tamkang Univ, Dept Math, New Taipei City 25137, Taiwan
[3] Nagasaki Univ, Atom Bomb Dis Inst, Stem Cell Biol Lab, Nagasaki, Japan
[4] Natl Chia Yi Univ, Dept Appl Chem, Chiayi 600, Taiwan
[5] 151 Ying Chuan Rd, New Taipei City 251, Taiwan
来源
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY | 2024年 / 278卷
关键词
Angiogenesis; Chick; Coumarin; Chick chorioallantoic membrane assay; VASCULAR DEVELOPMENT; PYRANOCOUMARINS; NEUROPILIN-1;
D O I
10.1016/j.cbpc.2024.109862
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Coumarin is a natural compound that is rich in plants. Coumarin and its derivates were reported to have many biological activities, such as anti-bacterial, anti-tumor, and anti-coagulation. In this study, we examined the angiogenic modulating activities of six previously synthesized coumarin derivatives (Compound #1-#6) in zebrafish embryos and further confirmed them in a chick model. According to the survival rate in a zebrafish model, Compound #1 (100 %), #2 (82.5-100 %), and #4 (100 %) showed much less toxicity than Compound #3 (19.2-100 %), #5 (0-100 %), and #6 (0-100 %). Using a green blood vessel fluorescent transgenic fish Tg(fli1: egfp) to record the angiogenesis-modulating effects of Compound #1, #2, and #4, we found that Compound #2 had the highest effects in interfering intersegmental vessel growth, subintestinal vein growth, and caudal vein plexus remodeling. Chick chorioallantoic membrane (CAM) assay also showed that Compound #2 exposure led to a reduction of blood vessel growth. Real-time PCR experiments revealed that Compound #2 significantly changed the expression of vascular growth-related genes flt1, cdh5, and nrp1a in zebrafish. Based on our data from zebrafish and chick models, a new coumarin-derivative (Compound #2) possesses anti-angiogenic activity with low toxicity, but further investigation in mammal models is asked to confirm our findings.
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页数:7
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