Myricetin attenuates hypoxia-induced inflammation in human adipocytes

被引:0
作者
Geiger, Kathrin [1 ,2 ]
Muendlein, Axel [1 ]
Leiherer, Andreas [1 ,2 ,3 ]
Gaenger, Stella [1 ]
Brandtner, Eva Maria [1 ]
Wabitsch, Martin [4 ]
Fraunberger, Peter [2 ]
Drexel, Heinz [1 ,3 ,5 ,6 ]
Heinzle, Christine [1 ,2 ]
机构
[1] Vorarlberg Inst Vasc Invest & Treatment VIVIT, Feldkirch, Austria
[2] Med Cent Labs, Feldkirch, Austria
[3] Private Univ Principal Liechtenstein, Triesen, Liechtenstein
[4] Univ Med Ctr Ulm, Dept Pediat & Adolescent Med, Div Pediat Endocrinol & Diabet, Ulm, Germany
[5] Acad Teaching Hosp Feldkirch, Vorarlberger Landeskrankenhausbetriebsgesellschaft, Feldkirch, Austria
[6] Drexel Univ, Coll Med, Philadelphia, PA USA
关键词
Myricetin; Hypoxia; Adipokines; HIF-1; alpha; NF-kappa B; SGBS adipocytes; NF-KAPPA-B; INSULIN-RESISTANCE; ADIPOSE-TISSUE; GENE-EXPRESSION; HIGH-FAT; OBESITY; PREADIPOCYTES; HIF-1-ALPHA; ACTIVATION; ADIPOKINES;
D O I
10.1007/s11033-023-08865-9
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Background:Adipose tissue hypoxia plays a crucial role in the development of chronic low-grade systemic inflammation which has been associated with the pathogenesis of obesity-related diseases. Myricetin is a natural compound present in numerous plant-based foods with presumed anti-inflammatory and beneficial health effects. The impact of this flavonoid on hypoxia-induced expression of inflammatory adipokines and hypoxia-regulated pathways is unknown so far and has been addressed in the present study. Methods:Differentiated human Simpson-Golabi-Behmel syndrome (SGBS) adipocytes were cultured with or without myricetin under normoxic and hypoxic conditions for varying time periods. The effect of hypoxia and myricetin on the expression of the investigated adipokines was measured by real-time RT-PCR. Western blot analysis was used for the detection of transcription factors involved in hypoxia-regulated pathways. Results:Myricetin interfered in the hypoxia-induced regulation of adipokines and the underlying pathways, which are involved in transmitting the inflammatory response. It strongly repressed hypoxia-induced expression of apelin, leptin, chemerin, asprosin, and DPP-4 and HIF-1 alpha accumulation in the nucleus was diminished. Furthermore, the activation of the key regulators in the inflammatory response NF-kappa B, Akt, and CREB was suppressed by myricetin under hypoxic conditions. Myricetin also decreased hypoxia-induced accumulation of the pro-tumorigenic transcription factors Snail and Slug in the nucleus. Conclusion:Taken together, our results indicated that myricetin regulated hypoxia-induced expression of adipokines and hypoxia-regulated pathways in human adipocytes. Our study therefore provided evidence of the anti-inflammatory effects of myricetin in hypoxia-treated human adipocytes.
引用
收藏
页码:9833 / 9843
页数:11
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