Efficient Metabolic Fingerprinting of Follicular Fluid Encodes Ovarian Reserve and Fertility

被引：7
作者：
Wu, Jiao [1 ,2 ,3 ,4 ,8 ]
Liang, Chunmei [6 ,7 ]
Wang, Xin
Huang, Yida [1 ,2 ,3 ,4 ]
Liu, Wanshan [1 ,2 ,3 ,4 ]
Wang, Ruimin [1 ,2 ,3 ,4 ]
Cao, Jing [1 ,2 ,3 ,4 ]
Su, Xun
Yin, Tao
Wang, Xiaolei
Zhang, Zhikang
Shen, Lingchao
Li, Danyang
Zou, Weiwei [5 ,6 ,7 ]
Wu, Ji [9 ]
Qiu, Lihua [1 ,10 ]
Di, Wen [1 ,10 ]
Cao, Yunxia [5 ,6 ,7 ]
Ji, Dongmei [5 ,6 ,7 ]
Qian, Kun [1 ,2 ,3 ,4 ]
机构：
[1] Shanghai Jiao Tong Univ, Renji Hosp, Sch Med, Dept Obstet & Gynecol,Shanghai Key Lab Gynecol Onc, Shanghai 200127, Peoples R China
[2] Shanghai Jiao Tong Univ, Sch Biomed Engn, State Key Lab Syst Med Canc, Shanghai 200030, Peoples R China
[3] Shanghai Jiao Tong Univ, Inst Med Robot, Shanghai 200030, Peoples R China
[4] Shanghai Jiao Tong Univ, Renji Hosp, Sch Med, Div Cardiol, Shanghai 200127, Peoples R China
[5] Anhui Med Univ, Affiliated Hosp 1, Dept Obstet & Gynecol, Hefei 230022, Peoples R China
[6] Anhui Med Univ, NHC Key Lab Study Abnormal Gametes & Reprod Tract, Hefei 230032, Peoples R China
[7] Anhui Med Univ, Key Lab Populat Hlth Life Cycle, Hefei 230032, Peoples R China
[8] Shanghai Jiao Tong Univ, Renji Hosp, Biox Inst, Sch Med,Key Lab Genet Dev & Neuropsychiat Disorder, Shanghai 200240, Peoples R China
[9] Ningxia Med Univ, Key Lab Fertil Preservat & Maintenance, Minist Educ, Yinchuan 750003, Peoples R China
[10] Shanghai Jiao Tong Univ, Ren Ji Hosp, Sch Med, Dept Obstet & Gynecol, Shanghai 200127, Peoples R China
来源：
ADVANCED SCIENCE | 2023年 / 10卷 / 23期
基金：
国家重点研发计划;
关键词：
biofluids; biomarkers; diagnostics; mass spectrometry; metabolism; SERUM; POPULATION; PLASMA; URINE;
D O I：
10.1002/advs.202302023
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Ovarian reserve (OR) and fertility are critical in women's healthcare. Clinical methods for encoding OR and fertility rely on the combination of tests, which cannot serve as a multi-functional platform with limited information from specific biofluids. Herein, metabolic fingerprinting of follicular fluid (MFFF) from follicles is performed, using particle-assisted laser desorption/ionization mass spectrometry (PALDI-MS) to encode OR and fertility. PALDI-MS allows efficient MFFF, showing fast speed (approximate to 30 s), high sensitivity (approximate to 60 fmol), and desirable reproducibility (coefficients of variation <15%). Further, machine learning of MFFF is applied to diagnose diminished OR (area under the curve of 0.929) and identify high-quality oocytes/embryos (p < 0.05) by a single PALDI-MS test. Meanwhile, metabolic biomarkers from MFFF are identified, which also determine oocyte/embryo quality (p < 0.05) from the sampling follicles toward fertility prediction in clinics. This approach offers a powerful platform in women's healthcare, not limited to OR and fertility.
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页数：11
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共 56 条
  • [1] Silver-109-based laser desorption/ionization mass spectrometry method for detection and quantification of amino acids
    Arendowski, Adrian
    Niziol, Joanna
    Ruman, Tomasz
    [J]. JOURNAL OF MASS SPECTROMETRY, 2018, 53 (04): : 369 - 378
  • [2] Which is more predictive ovarian sensitivity marker if there is discordance between serum anti-Mullerian hormone levels and antral follicle count? A retrospective analysis
    Aslan, Kiper
    Kasapoglu, Isil
    Cakir, Cihan
    Koc, Meltem
    Celenk, Murat Deniz
    Ata, Baris
    Avci, Berrin
    Uncu, Gurkan
    [J]. JOURNAL OF OBSTETRICS AND GYNAECOLOGY, 2022, 42 (05) : 1461 - 1466
  • [3] Metabolic profiling, metabolomic and metabonomic procedures for NMR spectroscopy of urine, plasma, serum and tissue extracts
    Beckonert, Olaf
    Keun, Hector C.
    Ebbels, Timothy M. D.
    Bundy, Jacob G.
    Holmes, Elaine
    Lindon, John C.
    Nicholson, Jeremy K.
    [J]. NATURE PROTOCOLS, 2007, 2 (11) : 2692 - 2703
  • [4] Added value of ovarian reserve testing on patient characteristics in the prediction of ovarian response and ongoing pregnancy: an individual patient data approach
    Broer, Simone L.
    van Disseldorp, Jeroen
    Broeze, Kimiko A.
    Dolleman, Madeleine
    Opmeer, Brent C.
    Bossuyt, Patrick
    Eijkemans, Marinus J. C.
    Mol, Ben-Willem J.
    Broekmans, Frank J. M.
    Anderson, R. A.
    Ashrafi, M.
    Bancsi, L.
    Caroppo, E.
    Copperman, A.
    Ebner, T.
    Geva, Eldar M.
    Erdem, M.
    Greenblatt, E. M.
    Jayaprakasan, K.
    Fenning, Raine
    Klinkert, E. R.
    Kwee, J.
    Lambalk, C. B.
    La Marca, A.
    McIlveen, M.
    Merce, L. T.
    Muttukrishna, S.
    Nelson, S. M.
    Ng, H. Y.
    Popovic-Todorovic, B.
    Smeenk, J. M. J.
    Tomas, C.
    Van der Linden, P. J. Q.
    van Rooij, I. A.
    Vladimirov, I. K.
    Bossuyt, P. B.
    Eijkemans, M. J. C.
    Mol, B. W.
    Broekmans, Frank
    [J]. HUMAN REPRODUCTION UPDATE, 2013, 19 (01) : 26 - 36
  • [5] Metabolomic profiles predict individual multidisease outcomes
    Buergel, Thore
    Steinfeldt, Jakob
    Ruyoga, Greg
    Pietzner, Maik
    Bizzarri, Daniele
    Vojinovic, Dina
    zu Belzen, Julius Upmeier
    Loock, Lukas
    Kittner, Paul
    Christmann, Lara
    Hollmann, Noah
    Strangalies, Henrik
    Braunger, Jana M.
    Wild, Benjamin
    Chiesa, Scott T.
    Spranger, Joachim
    Klostermann, Fabian
    van den Akker, Erik B.
    Trompet, Stella
    Mooijaart, Simon P.
    Sattar, Naveed
    Jukema, J. Wouter
    Lavrijssen, Birgit
    Kavousi, Maryam
    Ghanbari, Mohsen
    Ikram, Mohammad A.
    Slagboom, Eline
    Kivimaki, Mika
    Langenberg, Claudia
    Deanfield, John
    Eils, Roland
    Landmesser, Ulf
    [J]. NATURE MEDICINE, 2022, 28 (11) : 2309 - +
  • [6] Emerging Applications of Mass Spectrometry-Based Metabolic Fingerprinting in Clinics
    Cao, Jing
    Wang, Yuning
    Zhang, Yong
    Qian, Kun
    [J]. ADVANCED INTELLIGENT SYSTEMS, 2022, 4 (05)
  • [7] Metabolic Fingerprinting on Synthetic Alloys for Medulloblastoma Diagnosis and Radiotherapy Evaluation
    Cao, Jing
    Shi, Xuejiao
    Gurav, Deepanjali D.
    Huang, Lin
    Su, Haiyang
    Li, Keke
    Niu, Jingyang
    Zhang, Mengji
    Wang, Qian
    Jiang, Mawei
    Qian, Kun
    [J]. ADVANCED MATERIALS, 2020, 32 (23)
  • [8] Rapid electrochemical detection of coronavirus SARS-CoV-2
    Chaibun, Thanyarat
    Puenpa, Jiratchaya
    Ngamdee, Tatchanun
    Boonapatcharoen, Nimaradee
    Athamanolap, Pornpat
    O'Mullane, Anthony Peter
    Vongpunsawad, Sompong
    Poovorawan, Yong
    Lee, Su Yin
    Lertanantawong, Benchaporn
    [J]. NATURE COMMUNICATIONS, 2021, 12 (01)
  • [9] Diminished ovarian reserve, premature ovarian failure, poor ovarian responder-a plea for universal definitions
    Cohen, J.
    Chabbert-Buffet, N.
    Darai, E.
    [J]. JOURNAL OF ASSISTED REPRODUCTION AND GENETICS, 2015, 32 (12) : 1709 - 1712
  • [10] Targeted metabolomics reveals reduced levels of polyunsaturated choline plasmalogens and a smaller dimethylarginine/arginine ratio in the follicular fluid of patients with a diminished ovarian reserve
    de la Barca, J. M. Chao
    Boueilh, T.
    Simard, G.
    Boucret, L.
    Ferre-L'Hotellier, V.
    Tessier, L.
    Gadras, C.
    Bouet, P. E.
    Descamps, P.
    Procaccio, V.
    Reynier, P.
    May-Panloup, P.
    [J]. HUMAN REPRODUCTION, 2017, 32 (11) : 2269 - 2278
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