Semi-quantitative Determination of Protein Expression Using Immunohistochemistry Staining and Analysis

被引：13

作者：

Crowe, Alexandra R. ^{[1
]}

Yue, Wei ^{[1
]}

机构：

[1] Univ Oklahoma, Coll Pharm, Dept Pharmaceut Sci, Hlth Sci Ctr, Oklahoma City, OK 73019 USA

来源：

BIO-PROTOCOL | 2023年 / 13卷 / 02期

关键词：

Immunohistochemistry; Semi; -quantification; Fiji ImageJ; DAB staining; Hematoxylin; QUANTITATIVE IMMUNOHISTOCHEMISTRY; QUANTIFICATION;

D O I：

10.21769/BioProtoc.4610

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Semi-quantitative immunohistochemistry (IHC) is a powerful method for investigating protein expression and localization within tissues that involves using software, such as the freely available Fiji (ImageJ), to conduct deconvolution and downstream analysis. Currently, there is lack of an integrated protocol that includes a detailed procedure on how to measure or compare protein expression. Publications that use semi-quantitative methods to ascertain protein expression often don't provide enough details in their methods section, which makes it difficult for the reader to reproduce their data. The current protocol provides an example and detailed steps of conducting semi-quantitative analysis of IHC images using Fiji software.

引用

页数：11

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