Transcriptome Sequencing Reveals Key Genes for Sunflower Unsaturated Fatty Acid Synthesis

被引:3
作者
Huang, Qixiu [1 ,2 ]
Xiang, Lijun [2 ]
Zhang, Li [2 ]
Maimaiti, Yushanjiang [1 ]
Luo, Wenfang [1 ]
Lei, Zhonghua [1 ,2 ]
机构
[1] Xinjiang Acad Agr Sci, Inst Plant Protect, Lab Integrated Pest Management Crops Northwestern, Minist Agr & Rural Affairs, Urumqi 830091, Peoples R China
[2] Xinjiang Acad Agr Sci, Inst Econ Crops, Urumqi 830091, Peoples R China
来源
AGRONOMY-BASEL | 2023年 / 13卷 / 03期
关键词
sunflower; transcriptome; fatty acids; unsaturated fatty acids; oleic acids; linoleic acids; VEGETABLE-OILS; FAD2; ACCUMULATION; EXPRESSION; STRESS;
D O I
10.3390/agronomy13030885
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
Sunflower (Helianthus annuus L.) is an important oil crop with rich nutrients, and genetically engineered breeding has become an important way to improve its quality. In this study, five varieties of oilseed sunflower were analyzed for fatty acid (FA) content. The seed embryos of one of the high oleic acid (OA) varieties were transcriptome sequenced at different stages. The results showed that OA synthesis dominated the unsaturated fatty acid (UFA) synthesis pathways in seed embryos. Substantially differentially expressed genes were detected at various post-flowering stages. Specifically, the up-regulated gene numbers were highest at 10 d after flowering, while most genes were down-regulated at 20 d after flowering. The enriched genes were rather consistent with almost all experimental groups exhibiting enrichment to the FAD2 gene. The expression of FAD2 was highly negatively correlated with the expressions of FAD6, FAD3, and FAD7. During seed embryo development, the expression level of FAD2 was highly negatively correlated with the final OA content and was highly positively correlated with the final linoleic acid (LA) content. This suggests that the FAD2 is a key enzyme catalyzing the OA to LA conversion.
引用
收藏
页数:17
相关论文
共 54 条
[1]   Genotype and Seasonal Variation Affect Yield and Oil Quality of Safflower (Carthamus tinctorius L.) under Mediterranean Conditions [J].
Abou Chehade, Lara ;
Angelini, Luciana G. ;
Tavarini, Silvia .
AGRONOMY-BASEL, 2022, 12 (01)
[2]   Prediction of fatty acid composition of sunflower seeds by near-infrared reflectance spectroscopy [J].
Akkaya, Murat Reis .
JOURNAL OF FOOD SCIENCE AND TECHNOLOGY-MYSORE, 2018, 55 (06) :2318-2325
[3]   Microbial Synthesis of Linoleate 9S-Lipoxygenase Derived Plant C18 Oxylipins from C18 Polyunsaturated Fatty Acids [J].
An, Jung-Ung ;
Lee, In-Gyu ;
Ko, Yoon-Joo ;
Oh, Deok-Kun .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2019, 67 (11) :3209-3219
[4]   Dehydrins in Orthodox and Recalcitrant Seeds [J].
Azarkovich, M. I. .
RUSSIAN JOURNAL OF PLANT PHYSIOLOGY, 2020, 67 (02) :221-230
[5]   The sunflower genome provides insights into oil metabolism, flowering and Asterid evolution [J].
Badouin, Helene ;
Gouzy, Jerome ;
Grassa, Christopher J. ;
Murat, Florent ;
Staton, S. Evan ;
Cottret, Ludovic ;
Lelandais-Briere, Christine ;
Owens, Gregory L. ;
Carrere, Sebastien ;
Mayjonade, Baptiste ;
Legrand, Ludovic ;
Gill, Navdeep ;
Kane, Nolan C. ;
Bowers, John E. ;
Hubner, Sariel ;
Bellec, Arnaud ;
Berard, Aurelie ;
Berges, Helene ;
Blanchet, Nicolas ;
Boniface, Marie-Claude ;
Brunel, Dominique ;
Catrice, Olivier ;
Chaidir, Nadia ;
Claudel, Clotilde ;
Donnadieu, Cecile ;
Faraut, Thomas ;
Fievet, Ghislain ;
Helmstetter, Nicolas ;
King, Matthew ;
Knapp, Steven J. ;
Lai, Zhao ;
Le Paslier, Marie-Christine ;
Lippi, Yannick ;
Lorenzon, Lolita ;
Mandel, Jennifer R. ;
Marage, Gwenola ;
Marchand, Gwenaelle ;
Marquand, Elodie ;
Bret-Mestries, Emmanuelle ;
Morien, Evan ;
Nambeesan, Savithri ;
Thuy Nguyen ;
Pegot-Espagnet, Prune ;
Pouilly, Nicolas ;
Raftis, Frances ;
Sallet, Erika ;
Schiex, Thomas ;
Thomas, Justine ;
Vandecasteele, Celine ;
Vares, Didier .
NATURE, 2017, 546 (7656) :148-+
[6]   Spatial and temporal expressions of two distinct oleate desaturases from olive (Olea europaea L.) [J].
Banilas, G ;
Moressis, A ;
Nikoloudakis, N ;
Hatzopoulos, P .
PLANT SCIENCE, 2005, 168 (02) :547-555
[7]   Comparative Analysis and Structural Modeling of Elaeis oleifera FAD2, a Fatty Acid Desaturase Involved in Unsaturated Fatty Acid Composition of American Oil Palm [J].
Ben Ayed, Rayda ;
Chirmade, Tejas ;
Hanana, Mohsen ;
Khamassi, Khalil ;
Ercisli, Sezai ;
Choudhary, Ravish ;
Kadoo, Narendra ;
Karunakaran, Rohini .
BIOLOGY-BASEL, 2022, 11 (04)
[8]   The cold resistance of cottonseed during germination is related to the content of unsaturated fatty acids [J].
Chen, Ying ;
Tian, Liwen ;
Wan, Lu ;
Xia, Jun ;
Liu, Feng ;
Zhao, Ruihai ;
Wang, Tangang ;
Han, Chunli .
CROP SCIENCE, 2021, 61 (05) :3598-3609
[9]   Genotyping and lipid profiling of 601 cultivated sunflower lines reveals novel genetic determinants of oil fatty acid content [J].
Chernova, Alina, I ;
Gubaev, Rim F. ;
Singh, Anupam ;
Sherbina, Katrina ;
Goryunova, Svetlana, V ;
Martynova, Elena U. ;
Goryunov, Denis, V ;
Boldyrev, Stepan, V ;
Vanyushkina, Anna A. ;
Anikanov, Nikolay A. ;
Stekolshchikova, Elena A. ;
Yushina, Ekaterina A. ;
Demurin, Yakov N. ;
Mukhina, Zhanna M. ;
Gavrilova, Vera A. ;
Anisimova, Irina N. ;
Karabitsina, Yulia, I ;
Alpatieva, Natalia, V ;
Chang, Peter L. ;
Khaitovich, Philipp ;
Mazin, Pavel, V ;
Nuzhdin, Sergey, V .
BMC GENOMICS, 2021, 22 (01)
[10]   Biological Role of Unsaturated Fatty Acid Desaturases in Health and Disease [J].
Czumaj, Aleksandra ;
Sledzinski, Tomasz .
NUTRIENTS, 2020, 12 (02)