Combined effect of electrical energy and graphene oxide on Enterococcus faecalis biofilms

被引：1
作者：
Lee, Myung-Jin [1 ]
Kim, Mi-Ah [2 ,3 ]
Min, Kyung-San [2 ,3 ,4 ,5 ]
机构：
[1] Seoul Natl Univ, Sch Dent, Dept Conservat Dent, Seoul 03080, South Korea
[2] Jeonbuk Natl Univ, Sch Dent, Dept Conservat Dent, Jeonju 54896, South Korea
[3] Jeonbuk Natl Univ, Inst Oral Biosci, Jeonju 54896, South Korea
[4] Jeonbuk Natl Univ, Res Inst Clin Med, Jeonju 54907, South Korea
[5] Jeonbuk Natl Univ Hosp, Biomed Res Inst, Jeonju 54907, South Korea
来源：
DENTAL MATERIALS JOURNAL | 2023年 / 42卷 / 06期
基金：
新加坡国家研究基金会;
关键词：
Biofilm; Electrical energy; Enterococcus faecalis; Graphene oxide; ENDODONTIC TREATMENT; BACTERIAL; ADHESION; EFFICACY; THERAPY; TEETH;
D O I：
10.4012/dmj.2023-087
中图分类号：
R78 [口腔科学];
学科分类号：
1003 ;
摘要：
This study aimed to investigate the effects of electrical energy and its synergistic activity with graphene oxide (GO) in Enterococcus faecalis (E. faecalis) biofilms. The viability of E. faecalis biofilms was analyzed by colony-forming units, crystal violet staining, and confocal laser scanning microscopy. The morphologies of the biofilms and the bacterial organelles were observed by scanning electron microscopy and transmission emission microscopy (TEM), respectively. Application of electrical energy combined with 0.2% sodium hypochlorite (NaOCl) on E. faecalis in biofilms significantly decreased the bacterial viability and biofilm biomass compared to the 0.2% NaOCl-only-treated group. Furthermore, additional application of GO showed similar antibacterial effects to 0.5% NaOCl. Notably, TEM observation revealed that the bacteria treated with electric energy and GO showed damaged cell membranes. The results suggest that combination of electrical energy and GO enhances antibacterial activity of NaOCl and has the potential to be applied to root canal irrigation protocols.
引用
收藏
页码：844 / 850
页数：7
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