Biological activity and inhibition potential against ?-glucosidase and ?-amylase of 2,4-di-tert-butylphenol from bamboo shoot extract by in vitro and in silico studies

We demonstrated the antioxidant activity, inhibitory activity against alpha-glucosidase, alpha-amylase, and the content of phenolic and flavonoid compounds from fresh and boiled bamboo shoot extracts. This research also first in-vestigates the content of 2,4-DTBP from bamboo shoots and its kinetic mechanism against alpha-glucosidase and alpha-amylase through in vitro and in silico studies. Total phenolic and flavonoid content and antioxidant activity of fresh samples were higher than that of boiled samples. Inhibitory activity of bamboo shoot extracts against alpha-glucosidase and alpha-amylase was related to the content of 2,4-DTBP by the highest potent obtained from the fresh samples. 2,4-DTBP displays mixed-type inhibition and is located at + 1 to + 2 subsites for alpha-glucosidase and-1 to-3 subsites for alpha-amylase. Our findings indicate that bamboo shoots had the source of bioactive compounds related to human health and the 2,4-DTBP in bamboo shoots is a candidate compound for anti-diabetes-related enzymes.