Comprehensive cell atlas of the first-trimester developing human brain

被引:79
作者
Braun, Emelie [1 ]
Danan-Gotthold, Miri [1 ,5 ]
Borm, Lars E. [1 ]
Lee, Ka Wai [1 ]
Vinsland, Elin [1 ]
Lonnerberg, Peter
Hu, Lijuan
Li, Xiaofei [2 ,3 ]
He, Xiaoling
Andrusivova, Zaneta [4 ]
Lundeberg, Joakim [4 ]
Barker, Roger A. [3 ]
Arenas, Ernest [1 ]
Sundstrom, Erik [2 ]
Linnarsson, Sten [1 ]
机构
[1] Karolinska Inst, Dept Med Biochem & Biophys, Div Mol Neurobiol, S-17165 Stockholm, Sweden
[2] Karolinska Inst, Dept Neurobiol Care Sci & Soc, Div Neurogeriatr, S-17165 Stockholm, Sweden
[3] Univ Cambridge, John Van Geest Ctr Brain Repair, Cambridge CB2 0PY, England
[4] KTH Royal Inst Technol, Dept Gene Technol, Sci Life Lab, S-17165 Solna, Sweden
[5] Weizmann Inst Sci, Dept Mol Genet, IL-7610001 Rehovot, Israel
基金
瑞典研究理事会; 欧盟地平线“2020”;
关键词
D O I
10.1126/science.adf1226
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
The adult human brain comprises more than a thousand distinct neuronal and glial cell types, a diversity that emerges during early brain development. To reveal the precise sequence of events during early brain development, we used single-cell RNA sequencing and spatial transcriptomics and uncovered cell states and trajectories in human brains at 5 to 14 postconceptional weeks (pcw). We identified 12 major classes that are organized as ~600 distinct cell states, which map to precise spatial anatomical domains at 5 pcw. We described detailed differentiation trajectories of the human forebrain and midbrain and found a large number of region-specific glioblasts that mature into distinct pre-astrocytes and pre–oligodendrocyte precursor cells. Our findings reveal the establishment of cell types during the first trimester of human brain development. © 2023 American Association for the Advancement of Science. All rights reserved.
引用
收藏
页码:172 / +
页数:15
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