Prognostic Impact of Stromal Profiles Educated by Gastric Cancer

被引：0

作者：

Takahashi, Kazunori ^{[1
]}

Shoda, Katsutoshi ^{[1
]}

Takiguchi, Koichi ^{[1
]}

Higuchi, Yudai ^{[1
]}

Matsuoka, Koichi ^{[1
]}

Nakayama, Takashi ^{[1
]}

Saito, Ryo ^{[1
]}

Maruyama, Suguru ^{[1
]}

Nakata, Yuki ^{[1
]}

Furuya, Shinji ^{[1
]}

Shiraishi, Kensuke ^{[1
]}

Akaike, Hidenori ^{[1
]}

Kawaguchi, Yoshihiko ^{[1
]}

Amemiya, Hidetake ^{[1
]}

Kawaida, Hiromichi ^{[1
]}

Ichikawa, Daisuke ^{[1
]}

机构：

[1] Univ Yamanashi, Dept Surg 1, Fac Med, Chuo, Yamanashi, Japan

来源：

ANNALS OF SURGICAL ONCOLOGY | 2024年 / 31卷 / 04期

关键词：

Cancer-associated fibroblast; Gastric cancer; Prognosis; Microenvironment; MESENCHYMAL STEM-CELLS; PODOPLANIN EXPRESSION; PLATELET-AGGREGATION; FIBROBLASTS; MYOFIBROBLASTS; ADENOCARCINOMA; IDENTIFICATION; PROGRESSION; CARCINOMA;

D O I：

10.1245/s10434-024-14888-8

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Background. Cancer-associated fibroblasts exhibit diversity and have several subtypes. The underlying relationship between the diversity of cancer-associated fibroblasts and their effect on gastric cancer progression remains unclear. In this study, mesenchymal stem cells were differentiated into cancer-associated fibroblasts with gastric cancer cell lines; clinical specimens were used to further investigate the impact of cancer-associated fibroblast diversity on cancer progression. Methods. Nine gastric cancer cell lines (NUGC3, NUGC4, MKN7, MKN45, MKN74, FU97, OCUM1, NCI-N87, and KATOIII) were used to induce mesenchymal stem cell differentiation into cancer-associated fibroblasts. The cancer-associated fibroblasts were classified based on ACTA2 and PDPN expression. Cell function analysis was used to examine the impact of cancer-associated fibroblast subtypes on cancer cell phenotype. Tissue samples from 97gastric patients who underwent gastrectomy were used to examine the clinical significance of each subtype classified according to cancer-associated fibroblast expression. Results. Co-culture of mesenchymal stem cells with nine gastric cancer cell lines revealed different subtypes of ACTA2 and PDPN expression in differentiated cancer-associated fibroblasts. Cancer-associated fibroblast subtypes with high ACTA2 plus PDPN expression levels significantly increased gastric cancer cell migration, invasion, and proliferation. The cancer-associated fibroblast subtype with ACTA2 plus PDPN expression was an independent prognostic factor along with lymph node metastasis for patients who had gastric cancer and were undergoing surgery. Conclusions. Cancer-associated fibroblasts are educated by gastric cancer cells during the development of cancer-associated fibroblast diversity. Differentiated cancer-associated fibroblasts with distinct expression patterns could affect gastric cancer progression and enable prognostic stratification for gastric cancer.

引用

页码：2309 / 2318

页数：10

共 44 条

[1]

[Anonymous], 2009, TNM Classification of malignant tumors

[2] The Role of Tumor Stroma in Cancer Progression and Prognosis Emphasis on Carcinoma-Associated Fibroblasts and Non-small Cell Lung Cancer [J].

Bremnes, Roy M. ;

Donnem, Tom ;

Al-Saad, Samer ;

Al-Shibli, Khalid ;

Andersen, Sigve ;

Sirera, Rafael ;

Camps, Carlos ;

Marinez, Inigo ;

Busund, Lill-Tove .

JOURNAL OF THORACIC ONCOLOGY, 2011, 6 (01) :209-217

[3] New horizons in tumor microenvironment biology: challenges and opportunities [J].

Chen, Fei ;

Zhuang, Xueqian ;

Lin, Liangyu ;

Yu, Pengfei ;

Wang, Ying ;

Shi, Yufang ;

Hu, Guohong ;

Sun, Yu .

BMC MEDICINE, 2015, 13

[4]

Cheng YF, 2015, INT J CLIN EXP MED, V8, P1896

[5] Cross-Species Single-Cell Analysis of Pancreatic Ductal Adenocarcinoma Reveals Antigen-Presenting Cancer-Associated Fibroblasts [J].

Elyada, Ela ;

Bolisetty, Mohan ;

Laise, Pasquale ;

Flynn, William F. ;

Courtois, Elise T. ;

Burkhart, Richard A. ;

Teinor, Jonathan A. ;

Belleau, Pascal ;

Biffi, Giulia ;

Lucito, Matthew S. ;

Sivajothi, Santhosh ;

Armstrong, Todd D. ;

Engle, Dannielle D. ;

Yu, Kenneth H. ;

Hao, Yuan ;

Wolfgang, Christopher L. ;

Park, Youngkyu ;

Preall, Jonathan ;

Jaffee, Elizabeth M. ;

Califano, Andrea ;

Robson, Paul ;

Tuveson, David A. .

CANCER DISCOVERY, 2019, 9 (08) :1102-1123

[6] Hypoxia, a key factor in the immune microenvironment [J].

Hu, Jingyao ;

Li, Xinyu ;

Yang, Liang ;

Li, Hangyu .

BIOMEDICINE & PHARMACOTHERAPY, 2022, 151

[7] Mechano-Signal Transduction in Mesenchymal Stem Cells Induces Prosaposin Secretion to Drive the Proliferation of Breast Cancer Cells [J].

Ishihara, Seiichiro ;

Inman, David R. ;

Li, Wan-Ju ;

Ponik, Suzanne M. ;

Keely, Patricia J. .

CANCER RESEARCH, 2017, 77 (22) :6179-6189

[8] Conservation of a platelet activating domain of Aggrus/podoplanin as a platelet aggregation-inducing factor [J].

Kaneko, Mika Kato ;

Kato, Yukinari ;

Kitano, Takashi ;

Osawa, Motoki .

GENE, 2006, 378 :52-57

[9] The role of cancer-associated fibroblasts, solid stress and other microenvironmental factors in tumor progression and therapy resistance [J].

Kharaishvili, Gvantsa ;

Simkova, Dana ;

Bouchalova, Katerina ;

Gachechiladze, Mariam ;

Narsia, Nato ;

Bouchal, Jan .

CANCER CELL INTERNATIONAL, 2014, 14

[10] Diffuse-type gastric cancer cells switch their driver pathways from FGFR2 signaling to SDF1/CXCR4 axis in hypoxic tumor microenvironments [J].

Kinoshita, Haruhito ;

Yashiro, Masakazu ;

Fukuoka, Tatsunari ;

Hasegawa, Tsuyoshi ;

Morisaki, Tamami ;

Kasashima, Hiroaki ;

Masuda, Go ;

Noda, Satoru ;

Hirakawa, Kosei .

CARCINOGENESIS, 2015, 36 (12) :1511-1520

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