Identification and functional comparison of novel alternatively spliced isoforms of human YAP

被引：0

作者：

Liu, Lianlian ^{[1
,2
]}

Zhang, Junlei ^{[1
]}

Wang, Jiaqi ^{[1
,3
]}

Tian, Yanping ^{[1
]}

Wang, Jiali ^{[1
]}

Xu, Yixiao ^{[1
]}

Cheng, Yuda ^{[1
,2
]}

Yu, Meng ^{[1
,4
]}

Wang, Jiangjun ^{[1
,5
]}

Yang, Yi ^{[6
]}

Wang, Xueyue ^{[7
]}

Yang, Ran ^{[1
,3
]}

Wu, Wei ^{[8
]}

Zhang, Chen ^{[1
]}

Hu, Yan ^{[9
]}

Jian, Rui ^{[1
,10
]}

Xiao, Lan ^{[2
,11
]}

Ruan, Yan ^{[1
,10
]}

机构：

[1] Army Med Univ, Dept Histol & Embryol, Lab Stem Cell & Dev Biol, Chongqing, Peoples R China

[2] Army Med Univ, Dept Histol & Embryol, Chongqing Key Lab Neurobiol Brain & Intelligence R, Key Lab Chongqing Educ Commiss, Chongqing, Peoples R China

[3] Army Med Univ, Coll High Altitude Mil Med, Dept Pathophysiol, Chongqing, Peoples R China

[4] Army Med Univ, Southwest Hosp, Joint Surg Ctr, Chongqing, Peoples R China

[5] Army Med Univ, Coll Basic Med, Dept Cell Biol, Chongqing, Peoples R China

[6] Army Med Univ, Coll Basic Med Sci, Expt Ctr Basic Med, Chongqing, Peoples R China

[7] Gen Hosp PLA Tibet Mil Area Command, Dept Pediat, Lhasa, Peoples R China

[8] Army Med Univ, Southwest Hosp, Thorac Surg Dept, Hosp 1, Chongqing, Peoples R China

[9] Army Med Univ, Dept Mil Basic Training & Army Management, Army Hlth Serv Training Base, Chongqing, Peoples R China

[10] Army Med Univ, Dept Histol & Embryol, Lab Stem Cell & Dev Biol, Chongqing 40038, Peoples R China

[11] Army Mil Med Univ, Dept Histol & Embryol, Chongqing Key Lab Neurobiol Brain & Intelligence R, Key Lab Chongqing Educ Commiss, Chongqing 40038, Peoples R China

来源：

FEBS OPEN BIO | 2023年 / 13卷 / 06期

基金：

中国国家自然科学基金;

关键词：

alternative splicing; cell chemosensitivity; transcriptional activation ability; YAP; YES-ASSOCIATED PROTEIN; HIPPO PATHWAY; TRANSCRIPTIONAL ACTIVATOR; NUCLEAR-LOCALIZATION; SIZE-CONTROL; P73; CANCER; DOMAIN; INDUCTION; BINDING;

D O I：

10.1002/2211-5463.13618

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

As a key effector of the Hippo pathway, yes-associated protein (YAP) is a major regulator of cell proliferation and apoptosis. In this study, 23 hYAP isoforms were identified in HEK293 cells, with 14 isoforms being reported for the first time. These isoforms were classified into hYAP-a and hYAP-b isoforms based on the variation in exon 1. The two groups of isoforms showed distinctly different subcellular localizations. hYAP-a isoforms could activate TEAD- or P73-mediated transcription, affect the proliferation rate, and enhance the cellular chemosensitivity of HEK293 cells. Moreover, different activation abilities and pro-cytotoxic effects were observed among hYAP-a isoforms. However, hYAP-b isoforms were not found to exert any significant biological effects. Our findings add to the knowledge of YAP gene structure and protein-coding capacity and will help in the elucidation of the function and related molecular mechanisms of the Hippo-YAP signaling pathway.

引用

页码：1001 / 1014

页数：14

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