OIL-SEALED RGD-MODIFIED HYDROGEL MICROWELL ARRAY WITH SIZE-SELECTIVE PERMEATION FOR ANALYSIS ON EXOSOMES FROM SINGLE CELLS

被引：0

作者：

Yamagata, Chisaki ^{[1
]}

Itai, Shun ^{[1
]}

Kurashina, Yuta ^{[2
]}

Asai, Makoto ^{[3
]}

Hoshino, Ayuko ^{[4
]}

Onoe, Hiroaki ^{[1
]}

机构：

[1] Keio Univ, Fac Sci & Technol, Kyoto, Japan

[2] Tokyo Univ Agr & Technol, Fac Engn, Tokyo, Japan

[3] Keio Univ, Global Res Inst, Kyoto, Japan

[4] Tokyo Inst Technol, Sch Life Sci & Technol, Tokyo, Japan

来源：

2023 IEEE 36TH INTERNATIONAL CONFERENCE ON MICRO ELECTRO MECHANICAL SYSTEMS, MEMS | 2023年

关键词：

Exosome; Extracellular vesicle; Single-cell analysis; Microwell array; Alginate; Hydrogel; Arginine-glycine-aspartate (RGD) motif;

D O I：

10.1109/MEMS49605.2023.10052318

中图分类号：

TM [电工技术]; TN [电子技术、通信技术];

学科分类号：

0808 ; 0809 ;

摘要：

We propose an oil-sealed arginine-glycine-aspartate (RGD)-modified hydrogel microwell array for analyzing exosomes secreted from single cells. Our device realizes the collection of exosomes from single cells in parallel. The size-selective permeation of the RGD-modified alginate hydrogel allows both stable cell culturing of single or few cells in closed wells and the confinement of exosomes secreted in each space. The nutrients (< 20 nm) that are necessary for cell culture can pass through the hydrogel, while exosomes (30-150 nm) do not permeate the walls of microwells. The cell culture property and the permeability of our device were examined, showing the capability to collect exosomes from single cells. We believe that our device would contribute to understanding the mechanisms of various diseases.

引用

页码：319 / 322

页数：4