CYP6DW3 Metabolizes Imidacloprid to Imidacloprid-urea in Whitefly (Bemisia tabaci)

被引：18

作者：

Xue, Hu ^{[1
,2
]}

Fu, Buli ^{[2
,3
]}

Huang, Mingjiao ^{[2
,4
]}

He, Chao ^{[2
]}

Liang, Jinjin ^{[2
]}

Yang, Jing ^{[2
]}

Wei, Xuegao ^{[1
,2
]}

Liu, Shaonan ^{[2
]}

Du, Tianhua ^{[2
]}

Ji, Yao ^{[2
]}

Yin, Cheng ^{[1
,2
]}

Gong, Peipan ^{[2
]}

Hu, JinYu ^{[1
,2
]}

Du, He ^{[2
]}

Zhang, Rong ^{[1
,2
]}

Wang, Chao ^{[1
,2
]}

Khajehali, Jahangir ^{[5
]}

Su, Qi ^{[1
]}

Yang, Xin ^{[2
]}

Zhang, Youjun ^{[1
,2
]}

机构：

[1] Yangtze Univ, Coll Agr, Hubei Engn Technol Ctr Pest Forewarning & Manageme, Jingzhou 434025, Hubei, Peoples R China

[2] Chinese Acad Agr Sci, Inst Vegetables & Flowers, State Key Lab Vegetable Biobreeding, Beijing 100081, Peoples R China

[3] Chinese Acad Trop Agr Sci, Environm & Plant Protect Inst, Key Lab Integrated Pest Management Trop Crops, Minist Agr & Rural Affairs, Haikou 571101, Peoples R China

[4] Hunan Agr Univ, Coll Plant Protect, Changsha 410125, Peoples R China

[5] Isfahan Univ Technol, Coll Agr, Dept Plant Protect, Esfahan 8415683111, Iran

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2023年 / 71卷 / 05期

基金：

北京市自然科学基金; 美国国家科学基金会; 中国国家自然科学基金;

关键词：

Bemisia tabaci; P450s; imidacloprid resistance; in vitro metabolism; pest management; NEONICOTINOID CROSS-RESISTANCE; INSECTICIDE RESISTANCE; NILAPARVATA-LUGENS; HEMIPTERA; CYTOCHROME-P450; EXPRESSION; HOMOPTERA; FIELD; PLANTHOPPER; ADAPTATION;

D O I：

10.1021/acs.jafc.2c08353

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

Bemisia tabaci has developed high resistance to many insecticides and causes substantial agricultural and economic losses annually. The insecticide resistance of whitefly has been widely reported in previous studies; however, the underlying mechanism remains little known. In this study, we cloned two P450 genes: CYP6DW3 and CYP6DW5v1; these genes were markedly overexpressed in imidacloprid-resistant whitefly populations compared with susceptible populations, and knockdown of these genes decreased the imidacloprid resistance of whitefly. Moreover, heterologous expression of whitefly P450 genes in SF9 cells and metabolic studies showed that the CYP6DW3 protein could metabolize 14.11% imidacloprid and produced imidacloprid-urea in vitro. Collectively, the expression levels of CYP6DW3 and CYP6DW5v1 are positively correlated with imidacloprid resistance in B. tabaci. Our study further reveals that cytochrome P450 enzymes affect the physiological activities related to resistance in insects, which helps scholars more deeply understand the resistance mechanism, and contributes to the development of integrated pest management framework.

引用

页码：2333 / 2343

页数：11

共 22 条

[21] Role of miR-276-3p in the cyantraniliprole resistance mechanism of Bemisia tabaci via CYP6CX3 targeting
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2024, 254
[22] Knockdown of one cytochrome P450 gene CYP6DW4 increases the susceptibility of Bemisia tabaci to dimpropyridaz, a novel pyridazine pyrazolecarboxamide insecticide
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Luo, Chen
Wang, Ran
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