CYP6DW3 Metabolizes Imidacloprid to Imidacloprid-urea in Whitefly (Bemisia tabaci)

被引：18

作者：

Xue, Hu ^{[1
,2
]}

Fu, Buli ^{[2
,3
]}

Huang, Mingjiao ^{[2
,4
]}

He, Chao ^{[2
]}

Liang, Jinjin ^{[2
]}

Yang, Jing ^{[2
]}

Wei, Xuegao ^{[1
,2
]}

Liu, Shaonan ^{[2
]}

Du, Tianhua ^{[2
]}

Ji, Yao ^{[2
]}

Yin, Cheng ^{[1
,2
]}

Gong, Peipan ^{[2
]}

Hu, JinYu ^{[1
,2
]}

Du, He ^{[2
]}

Zhang, Rong ^{[1
,2
]}

Wang, Chao ^{[1
,2
]}

Khajehali, Jahangir ^{[5
]}

Su, Qi ^{[1
]}

Yang, Xin ^{[2
]}

Zhang, Youjun ^{[1
,2
]}

机构：

[1] Yangtze Univ, Coll Agr, Hubei Engn Technol Ctr Pest Forewarning & Manageme, Jingzhou 434025, Hubei, Peoples R China

[2] Chinese Acad Agr Sci, Inst Vegetables & Flowers, State Key Lab Vegetable Biobreeding, Beijing 100081, Peoples R China

[3] Chinese Acad Trop Agr Sci, Environm & Plant Protect Inst, Key Lab Integrated Pest Management Trop Crops, Minist Agr & Rural Affairs, Haikou 571101, Peoples R China

[4] Hunan Agr Univ, Coll Plant Protect, Changsha 410125, Peoples R China

[5] Isfahan Univ Technol, Coll Agr, Dept Plant Protect, Esfahan 8415683111, Iran

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2023年 / 71卷 / 05期

基金：

北京市自然科学基金; 美国国家科学基金会; 中国国家自然科学基金;

关键词：

Bemisia tabaci; P450s; imidacloprid resistance; in vitro metabolism; pest management; NEONICOTINOID CROSS-RESISTANCE; INSECTICIDE RESISTANCE; NILAPARVATA-LUGENS; HEMIPTERA; CYTOCHROME-P450; EXPRESSION; HOMOPTERA; FIELD; PLANTHOPPER; ADAPTATION;

D O I：

10.1021/acs.jafc.2c08353

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

Bemisia tabaci has developed high resistance to many insecticides and causes substantial agricultural and economic losses annually. The insecticide resistance of whitefly has been widely reported in previous studies; however, the underlying mechanism remains little known. In this study, we cloned two P450 genes: CYP6DW3 and CYP6DW5v1; these genes were markedly overexpressed in imidacloprid-resistant whitefly populations compared with susceptible populations, and knockdown of these genes decreased the imidacloprid resistance of whitefly. Moreover, heterologous expression of whitefly P450 genes in SF9 cells and metabolic studies showed that the CYP6DW3 protein could metabolize 14.11% imidacloprid and produced imidacloprid-urea in vitro. Collectively, the expression levels of CYP6DW3 and CYP6DW5v1 are positively correlated with imidacloprid resistance in B. tabaci. Our study further reveals that cytochrome P450 enzymes affect the physiological activities related to resistance in insects, which helps scholars more deeply understand the resistance mechanism, and contributes to the development of integrated pest management framework.

引用

页码：2333 / 2343

页数：11

共 22 条

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Zhang, Rong
Yang, Jing
Hu, Jinyu
Yang, Fengbo
Liang, Jinjin
Xue, Hu
Wei, Xuegao
Fu, Buli
Huang, Mingjiao
Du, He
Wang, Chao
Su, Qi
Yang, Xin
Zhang, Youjun
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY, 2024, 201
[2] Differential metabolism of imidacloprid and dinotefuran by Bemisia tabaci CYP6CM1 variants
Hamada, Akira
Wahl, Gregory D.
Nesterov, Alexandre
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Banba, Shinichi
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY, 2019, 159 : 27 - 33
[3] Expression profile of CYP402C1 and its role in resistance to imidacloprid in the whitefly, Bemisia tabaci
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INSECT SCIENCE, 2023, 30 (01) : 146 - 160
[4] Cytochrome P450 CYP6DB3 was involved in thiamethoxam and imidacloprid resistance in Bemisia tabaci Q (Hemiptera: Aleyrodidae)
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Hu, Jinyu
Yang, Jing
Yin, Cheng
Du, Tianhua
Huang, Mingjiao
Fu, Buli
Gong, Peipan
Liang, Jinjin
Liu, Shaonan
Xue, Hu
He, Chao
Ji, Yao
Du, He
Zhang, Rong
Wang, Chao
Li, Junkai
Yang, Xin
Zhang, Youjun
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY, 2023, 194
[5] Two cytochrome P450 genes are involved in imidacloprid resistance in field populations of the whitefly, Bemisia tabaci, in China
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Gong, Pei-Pan
Wei, Xue-Gao
Liu, Shao-Nan
Yang, Jing
Fu, Bu-Li
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Huang, Ming-Jiao
Du, Tian-Hua
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PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY, 2023, 194
[7] Residues of imidacloprid insecticide and its efficacy on whitefly, Bemisia tabaci (gennadius) in tomato plants
Abdel-razik, M. A. A.
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[8] Cytpchrome P450 CYP4G68 Is Associated with Imidacloprid and Thiamethoxam Resistance in Field Whitefly, Bemisia tabaci (Hemiptera: Gennadius)
Liang, Jinjin
Yang, Jing
Hu, Jinyu
Fu, Buli
Gong, Peipan
Du, Tianhua
Xue, Hu
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Huang, Mingjiao
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Ji, Yao
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AGRICULTURE-BASEL, 2022, 12 (04):
[9] CYP6CX2 and CYP6CX3 mediate thiamethoxam resistance in field whitefly, Bemisia tabaci (Hemiptera:Aleyrodidae)
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Fu, Buli
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Zhang, Chengjia
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Yin, Cheng
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Du, Tianhua
Liang, Jinjin
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[10] Cytochrome P450 CYP6EM1 Underpins Dinotefuran Resistance in the Whitefly Bemisia tabaci
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