Separation of Phycobiliprotein from Nostoc Commune by Using Ion-Exchange Membrane with Quaternary Amine

Phycobiliprotein (PB), which is produced by cyanobacteria, has potential applications as a valuable pharmaceutical product. In this study, PB was separated by using a nonwoven-fabric membrane (Rx-1) that contained quaternary amino groups. PB was extracted from a Nostoc commune, which is a type of microalga, by disrupting the cell walls by a freeze-thaw method. Two types of biomolecules, namely PB and other biomolecules X, were identified by gel permeation chromatography. Permeation of the PB-containing solution through Rx-1 increased the purity of the PB in the filtrate from 0.25 to 0.45. The adsorption constant (K-p) and maximum adsorbed amount of each protein on Rx-1 (q(max)) were determined by fitting the experimental data to a mathematical model. The obtained values for K-p and q(max) were used to simulate scaled-up treatment of a PB-containing solution. Permeation of a PB-containing solution (3.0 L at 1.2 x 10(-3) m(3)/h) through Rx-1 (volume of scale-up membrane, 8.0 x 10(-4) m(3)) enabled PB separation for 26 h. Further PB purification could be achieved by using the Rx-1 membrane in combination with precipitation with ammonium sulfate solution and an ultrafiltration membrane.