Dynamics of extracellular vesicle uptake by mural granulosa cells in mice

被引：2

作者：

Edure, Taichi ^{[1
]}

Matsuno, Yuta ^{[1
,2
]}

Matsushita, Kodai ^{[1
]}

Maruyama, Natsumi ^{[1
]}

Fujii, Wataru ^{[1
,3
]}

Naito, Kunihiko ^{[1
]}

Sugiura, Koji ^{[1
]}

机构：

[1] Univ Tokyo, Grad Sch Agr & Life Sci, Dept Anim Resource Sci, Lab Appl Genet, 1-1-1 Yayoi,Bunkyo Ku, Tokyo, Japan

[2] Setsuro Tech Inc, Tokushima, Japan

[3] Univ Tokyo, Grad Sch Agr & Life Sci, Dept Vet Med Sci, Tokyo, Japan

来源：

MOLECULAR REPRODUCTION AND DEVELOPMENT | 2024年 / 91卷 / 03期

基金：

日本学术振兴会;

关键词：

endocytosis; extracellular vesicles; granulosa cells; oocytes; MOUSE CUMULUS CELLS; MEDIATED ENDOCYTOSIS; PARACRINE FACTORS; EXOSOME UPTAKE; OOCYTE; EXPRESSION; RECEPTOR; INTERNALIZATION; COMMUNICATION; CAVEOLIN-1;

D O I：

10.1002/mrd.23737

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Extracellular vesicles (EVs) play an important role in the development and function of mammalian ovarian follicles. However, the mechanisms by which they are taken up by the follicular granulosa cells remain unclear. In addition, while oocytes play a pivotal role in follicular development, the possible interactions between oocyte-derived paracrine factors (ODPFs) and EV signals are unknown. Therefore, this study aimed to elucidate the mechanism of EV uptake and the effects of ODPFs on EV uptake by follicular somatic mural granulosa cells in mice. Fluorescence-labeled transferrin (TRF) and cholera toxin B (CTB), substrates for clathrin- and caveolae-mediated endocytosis, respectively, were taken up by mural granulosa cells in vitro. Their uptake was inhibited by Pitstop 2 and genistein, inhibitors of clathrin and caveolae pathways, respectively. Mural granulosa cells took up EVs, and this uptake was suppressed by Pitstop 2 and genistein. Moreover, ODPFs promoted the uptake of EVs and CTB, but not TRF, by mural granulosa cells. These results suggest that mural granulosa cells take up EVs through both clathrin- and caveolae-mediated endocytosis and that oocytes may promote caveolae-mediated endocytosis to facilitate the uptake of EVs.

引用

页数：13

共 56 条

[1] Cellular and extracellular vesicular origins of miRNAs within the bovine ovarian follicle [J].