Choosing a polarisation configuration for dynamic light scattering and laser speckle contrast imaging

被引:8
作者
Akther, Sonam [1 ]
Mikkelsen, Markus B. [1 ]
Postnov, Dmitry [1 ]
机构
[1] Aarhus Univ, Dept Clin Med, DK-8200 Aarhus, Denmark
关键词
CEREBRAL-BLOOD-FLOW; STROKE;
D O I
10.1364/BOE.507367
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Laser speckle contrast imaging (LSCI) is applied in various biomedical applications for full -field characterization of blood flow and tissue perfusion. The accuracy of the contrast interpretation and its conversion to the blood flow index depends on specific parameters of the optical system and scattering media. One such parameter is the polarisation of detected light, which is often adjusted to minimize specular reflections and image artefacts. The polarisation's effect on the detected light scattering dynamics and, therefore, the accuracy of LSCI data interpretation requires more detailed investigation. In this study, we used LSCI and Dynamic Light Scattering Imaging to evaluate the effects of the detected light polarisation when imaging perfusion in the mouse cortex. We found that cross -polarisation results in a shorter decorrelation time constant, a higher coherence degree and stronger dynamic scattering compared to the parallel -polarisation or no-polariser configurations. These results support the cross -polarisation configuration as the most optimal for brain cortex imaging and suggest against direct or calibrated comparisons between the contrast recordings made with different polarisation configurations. (c) 2023 Optica Publishing Group under the terms of the Optica Open Access Publishing Agreement
引用
收藏
页码:336 / 345
页数:10
相关论文
共 31 条
  • [1] Mapping functional connectivity using cerebral blood flow in the mouse brain
    Bergonzi, Karla M.
    Bauer, Adam Q.
    Wright, Patrick W.
    Culver, Joseph P.
    [J]. JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM, 2015, 35 (03) : 367 - 370
  • [2] Establishing the diffuse correlation spectroscopy signal relationship with blood flow
    Boas, David A.
    Sakadzic, Sava
    Selb, Juliette
    Farzam, Parisa
    Franceschini, Maria Angela
    Carp, Stefan A.
    [J]. NEUROPHOTONICS, 2016, 3 (03)
  • [3] Laser speckle contrast imaging in biomedical optics
    Boas, David A.
    Dunn, Andrew K.
    [J]. JOURNAL OF BIOMEDICAL OPTICS, 2010, 15 (01)
  • [4] Intrinsic brain activity triggers trigeminal meningeal afferents in a migraine model
    Bolay, H
    Reuter, U
    Dunn, AK
    Huang, ZH
    Boas, DA
    Moskowitz, MA
    [J]. NATURE MEDICINE, 2002, 8 (02) : 136 - 142
  • [5] Laser speckle contrast imaging: theoretical and practical limitations
    Briers, David
    Duncan, Donald D.
    Hirst, Evan
    Kirkpatrick, Sean J.
    Larsson, Marcus
    Steenbergen, Wiendelt
    Stromberg, Tomas
    Thompson, Oliver B.
    [J]. JOURNAL OF BIOMEDICAL OPTICS, 2013, 18 (06)
  • [6] Imaging of the skin microvascularization using spatially depolarized dynamic speckle
    Colin, Elise
    Plyer, Aurelien
    Golzio, Muriel
    Meyer, Nicolas
    Favre, Gilles
    Orlik, Xavier
    [J]. JOURNAL OF BIOMEDICAL OPTICS, 2022, 27 (04)
  • [7] Sensitivity of laser speckle contrast imaging to flow perturbations in the cortex
    Davis, Mitchell A.
    Gagnon, Louis
    Boas, David A.
    Dunn, Andrew K.
    [J]. BIOMEDICAL OPTICS EXPRESS, 2016, 7 (03): : 759 - 775
  • [8] Can laser speckle flowmetry be made a quantitative tool?
    Duncan, Donald D.
    Kirkpatrick, Sean J.
    [J]. JOURNAL OF THE OPTICAL SOCIETY OF AMERICA A-OPTICS IMAGE SCIENCE AND VISION, 2008, 25 (08) : 2088 - 2094
  • [9] Dynamic imaging of cerebral blood flow using laser speckle
    Dunn, AK
    Bolay, T
    Moskowitz, MA
    Boas, DA
    [J]. JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM, 2001, 21 (03) : 195 - 201
  • [10] FLOW VISUALIZATION BY MEANS OF SINGLE-EXPOSURE SPECKLE PHOTOGRAPHY
    FERCHER, AF
    BRIERS, JD
    [J]. OPTICS COMMUNICATIONS, 1981, 37 (05) : 326 - 330