Multicenter evaluation of light transmission platelet aggregation reagents: communication from the ISTH SSC Subcommittee on Platelet Physiology

被引：9

作者：

Alessi, Marie-Christine ^{[1
]}

Coxon, Carmen ^{[2
]}

Ibrahim-Kosta, Manal ^{[1
]}

Bacci, Monica ^{[3
]}

Voisin, Sophie ^{[4
]}

Rivera, Jose ^{[5
]}

Greinacher, Andreas ^{[6
]}

Raster, Johannes ^{[6
]}

Pulcinelli, Fabio ^{[7
]}

Devreese, Katrien M. J. ^{[8
]}

Mullier, Francois ^{[9
]}

Mccormick, Aine N. ^{[10
]}

Frontroth, Juan Pablo ^{[11
]}

Pouplard, Claire ^{[12
]}

Sachs, Ulrich J. ^{[13
]}

Diaz, Isabelle ^{[14
]}

Bermejo, Nuria ^{[15
]}

Camera, Marina ^{[16
]}

Fontana, Pierre ^{[17
,18
]}

Bauters, Anne ^{[19
]}

Stepanian, Alain ^{[20
,21
]}

Cozzi, Maria R. ^{[22
]}

Sveshnikova, Anastasia N. ^{[23
]}

Faille, Dorothee ^{[24
]}

Hollon, Wendy ^{[25
]}

Chitlur, Meera ^{[26
]}

Casonato, Alessandra ^{[27
]}

Lasne, Dominique ^{[28
]}

Lavenu-Bombled, Cecile ^{[29
]}

Fiore, Mathieu ^{[30
]}

Hamidou, Bello ^{[1
]}

Hurtaud-Roux, Marie-Francoise ^{[31
]}

Saultier, Paul ^{[1
]}

Goumidi, Louisa ^{[1
]}

Gresele, Paolo ^{[32
]}

Lordkipanidze, Marie ^{[33
]}

机构：

[1] Aix Marseille Univ, Ctr Reference Pathol Plaquettaires, Lab Hematol, C2VN,INRAE,INSERM, Marseille, France

[2] Natl Inst Biol Stand & Controls, Blanche Lane, Potters Bar, Herts, England

[3] IRCCS Humanitas Res Hosp, Ctr Thrombosis & Hemorrhag Dis, Milan, Italy

[4] CHU Toulouse, Lab Hematol, Toulouse, France

[5] Univ Murcia, Hosp Univ Morales Meseguer, Ctr Reg Hemodonac, Serv Hematol & Oncol Med,Inst Murciano Invest Bio, Murcia, Spain

[6] Univ Med Greifswald, Inst Transfus Med, Greifswald, Germany

[7] Sapienza Univ Rome, Dept Expt Med, Rome, Italy

[8] Univ Ghent, Ghent Univ Hosp, Dept Diagnost Sci, Coagulat Lab, Ghent, Belgium

[9] Catholic Univ Louvain, Namur Thrombosis & Hemostasis Ctr, CHU UCLouvain Namur, Yvoir, Belgium

[10] St Thomas Hosp, Haemostasis & Thrombosis Lab, Viapath Analyt, London, England

[11] Hosp Pediat Prof Dr Juan P Garrahan, Lab Hemostasia & Trombosis, Buenos Aires, Argentina

[12] Univ Tours, Univ Hosp Tours, Dept Hemostasis, Tours, France

[13] Giessen Univ Hosp, Dept Thrombosis & Haemostasis, Giessen, Germany

[14] Univ Hosp Montpellier, Lab Hematol, Montpellier, France

[15] Complejo Hosp Univ Caceres, Hosp San Pedro Alcantara, Serv Hematol, Caceres, Spain

[16] Ist Ric & Cura Carattere Sci, Ctr Cardiol Monzino, Milan, Italy

[17] Geneva Univ Hosp, Div Angiol & Haemostasis, Geneva, Switzerland

[18] Geneva Platelet Grp, Fac Med, Geneva, Switzerland

[19] Hosp Univ Ctr Lille, Hemostasis Unit, Lille, France

[20] Univ Paris Cite, Hosp Grp Lariboisiere Fernand Widal, AP HP, Hematol Lab, Paris, France

[21] Univ Paris Cite, Hosp Grp Lariboisiere Fernand Widal, AP HP, Thrombosis Unit, Paris, France

[22] Ctr Riferimento Oncol Aviano, Immunopathol & Canc Biomarkers Unit, Aviano, Italy

[23] Dmitry Rogachev Pediat Hematol & Immunol Hosp, Moscow, Russia

[24] CHU Bichat Claude Bernard, AP HP, Dept Hematol Biol, Paris, France

[25] Wayne State Univ, Childrens Hosp Michigan, Jeanne M Lusher Special Coagulat Lab, Detroit, MI USA

[26] Cent Michigan Univ, Childrens Hosp Michigan, Jeanne M Lusher Special Coagulat Lab, Detroit, MI USA

[27] Univ Padua, Chair Internal Med 1, Med Sch, Dept Med, Padua, Italy

[28] Hop Necker Enfants Malad, AP HP, Lab dHematol, Paris, France

[29] CHU Bicetre, AP HP, Ctr Ressources & Competences Filiere Sante Malad, Fac Med Paris Saclay,Serv Hematol Biol, Le Kremlin Bicetre, France

[30] Bordeaux Univ Hosp, Ctr Reference Pathol Plaquettaires, Lab Hematol, Pessac, France

[31] Hop Robert Debre, AP HP, Ctr Reference Pathol Plaquettaires, Paris, France

[32] Univ Perugia, Dept Med & Surg, Perugia, Italy

[33] Univ Montreal, Montreal Heart Inst, Fac Pharm, Res Ctr, Montreal, PQ, Canada

来源：

JOURNAL OF THROMBOSIS AND HAEMOSTASIS | 2023年 / 21卷 / 09期

关键词：

activators; aggregation; multicenter; platelets; standardization; FUNCTION DISORDERS; AGGREGOMETRY; DIAGNOSIS; CONSENSUS;

D O I：

10.1016/j.jtha.2023.05.027

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Background: Light transmission aggregation (LTA) is used widely by the clinical and research communities. Although it is a gold standard, there is a lack of interlaboratory harmonization. Objectives: The primary objective was to assess whether sources of activators (mainly adenosine diphosphate [ADP], collagen, arachidonic acid, epinephrine, and thrombin receptor activating peptide6) and ristocetin contribute to poor LTA reproducibility. The secondary objective was to evaluate interindividual variability of results to appreciate the distribution of normal values and consequently better interpret pathologic results.Methods: An international multicenter study involving 28 laboratories in which we compared LTA results obtained with center-specific activators and a comparator that we supplied. Results: We report variability in the potency (P) of activators in comparison with the comparator. Thrombin receptor activating peptide 6 (P, 1.32-2.68), arachidonic acid (P, 0.87-1.43), and epinephrine (P, 0.97-1.34) showed the greatest variability. ADP (P, 1.04-1.20) and ristocetin (P, 0.98-1.07) were the most consistent. The data highlighted clear interindividual variability, notably for ADP and epinephrine. Four profiles of responses were observed with ADP from high-responders, intermediate-responders, and low-responders. A fifth profile corresponding to nonresponders (5% of the individuals) was observed with epinephrine.Conclusion: Based on these data, the establishment and adoption of simple standardization principles should mitigate variability due to activator sources. The observation of huge interindividual variability for certain concentrations of activators should lead to a cautious interpretation before reporting a result as abnormal. Confidence can be taken from the fact that difference between sources is not exacerbated in patients treated with antiplatelet agents.

引用

页码：2596 / 2610

页数：15

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