CRISPR-based engineering of RNA viruses

被引：5
作者：
Nemudryi, Artem [1 ]
Nemudraia, Anna [1 ]
Nichols, Joseph E. [1 ]
Scherffius, Andrew M. [1 ]
Zahl, Trevor [1 ]
Wiedenheft, Blake [1 ]
机构：
[1] Montana State Univ, Dept Microbiol & Cell Biol, Bozeman, MT 59717 USA
来源：
SCIENCE ADVANCES | 2023年 / 9卷 / 37期
关键词：
DOUBLE-STRAND BREAKS; DNA; CLEAVAGE; SYSTEMS; ENDONUCLEASE; EXPRESSION; KNOCKDOWN; EFFECTOR; COMPLEX;
D O I：
10.1126/sciadv.adj8277
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
CRISPR RNA-guided endonucleases have enabled precise editing of DNA. However, options for editing RNA remain limited. Here, we combine sequence-specific RNA cleavage by CRISPR ribonucleases with programmable RNA repair to make precise deletions and insertions in RNA. This work establishes a recombinant RNA technology with immediate applications for the facile engineering of RNA viruses.
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页数：9
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