Allantolactone Enhances In Vitro Cytotoxicity and Apoptosis Induction Induced by Cisplatin In Human Gastric Carcinoma Cells

To increase the effectiveness of gastric cancer therapy and the prognosis, novel anticancer med-icines must be developed for the treatment of gastric carcinoma. The main purpose of the present research work was to study the synergistic effects of allantolactone on the cytotoxicity and apoptosis induction efficacy of cisplatin in human gastric cancer cells. Cell viability was evaluated by MTT assay while cell apoptosis measurements were carried out by fluorescence microscopy. Effects on cell cycle were measured by flow cy-tometry while as effects on cellular migration and invasion were analyzed by transwell migration assay. The findings showed that the combination of allantolactone with cisplatin had a stronger effect on reducing cancer cell proliferation and triggering apoptosis than either treatment alone. As indicated by fluorescence micros-copy utilising (acridine orange/propidium iodide) AO/PI double staining, the combination of allantolactone with cisplatin led to more effective apoptosis induction. Compared to single drug treatment, the combination of allantolactone with cisplatin significantly increased the number of cells in the G2/M phase suggestive of a G2/M phase arrest. Additionally, allantolactone in combination with cis-platin induced a more pronounced inhibitory effect on the cell migration and cell invasion. The current findings imply that using allantolactone in conjunction with cisplatin may be a promising clinical chemotherapeutic strategy for gastric carcinoma treatment.