CRISPR/Cas9 in the era of nanomedicine and synthetic biology

被引：11

作者：

Schmidt, Tiziana Julia Nadjeschda ^{[1
]}

Berarducci, Barbara ^{[1
]}

Konstantinidou, Soultana ^{[1
]}

Raffa, Vittoria ^{[1
]}

机构：

[1] Univ Pisa, Dept Biol, SS12 Abetone & Brennero 4, I-56127 Pisa, Italy

来源：

DRUG DISCOVERY TODAY | 2023年 / 28卷 / 01期

关键词：

Genome editing; Nanomedicine; Synthetic biology; Nanogenetics; INDUCIBLE CRISPR-CAS9 SYSTEM; PEPTIDE-MEDIATED DELIVERY; CAS9; PROTEIN; DNA CLEAVAGE; GENOMIC DNA; GUIDE RNA; TRANSCRIPTION; REPEATS; BASE; NANOPARTICLES;

D O I：

10.1016/j.drudis.2022.103375

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

The CRISPR/Cas system was first discovered as a defense mechanism in bacteria and is now used as a tool for precise gene-editing applications. Rapidly evolving, it is increasingly applied in therapeutics. However, concerns about safety, specificity, and delivery still limit its potential. In this context, we introduce the concept of nanogenetics and speculate how the rational engineering of the CRISPR/Cas machinery could advance the biomedical field. In nanogenetics, the advantages of traditional approaches of synthetic biology could be expanded by nanotechnology approaches, enabling the design of a new generation of intrinsically safe and specific genome-editing platforms.

引用

页码：9 / 14

页数：6

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