Neglected bacterial infections associated to bovine respiratory disease in lactating cows from high-yielding dairy cattle herds

被引:4
作者
Massi, Rodrigo Pelisson [1 ]
Lunardi, Michele [1 ,2 ]
Alfieri, Alice Fernandes [1 ,2 ]
Alfieri, Amauri Alcindo [1 ,2 ]
机构
[1] Univ Estadual Londrina, Dept Vet Prevent Med, Lab Anim Virol, Celso Garcia Cid Rd,Univ Campus,POB 10011, BR-86057970 Londrina, Parana, Brazil
[2] Univ Estadual Londrina, Dept Vet Prevent Med, Mol Biol Unit, Multiuser Anim Hlth Lab, Celso Garcia Cid Rd,Univ Campus,POB 10011, BR-86057970 Londrina, Parana, Brazil
关键词
Dairy cows; Bovine respiratory disease complex; Neglected etiology; Mycoplasma bovis; Histophilus somni; Pasteurella multocida; PCR ASSAYS; CALVES; IDENTIFICATION; MOLLICUTES; PNEUMONIA; PATHOGENS; GENE;
D O I
10.1007/s42770-023-01165-1
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Bovine respiratory disease (BRD) is a multifactorial and predominantly multietiological disease that affects dairy cattle herds worldwide, being more frequent in young animals. The occurrence of BRD was investigated in lactating cows from two high-yielding dairy herds in southern Brazil. To determine the etiology of the clinical cases of acute respiratory disease, nasal swab samples were collected from cows with clinical signs of BRD and evaluated using PCR and RT-PCR for nucleic acid detection of the main BRD etiological agents, including Mycoplasma bovis, Mannheimia haemolytica, Pasteurella multocida, Histophilus somni, bovine respiratory syncytial virus, bovine coronavirus, bovine viral diarrhea virus, bovine alphaherpesvirus 1, and bovine parainfluenza virus 3. Only three microorganisms (M. bovis, H. somni, and P. multocida) were identified in both single and mixed infections. We concluded that 40.0% of the cows were infected with M. bovis and 75.0% with H. somni in herd A. Considering both single and mixed infections, the analyses performed in herd B showed that 87.5%, 25.0%, and 50.0% of the cows were infected with M. bovis, H. somni, and P. multocida, respectively. M. bovis and H. somni are considered fastidious bacteria and laboratory diagnosis is neglected. Subsequently, most clinical cases of mycoplasmosis and histophilosis in cattle remain undiagnosed. This study demonstrates the importance of M. bovis and H. somni infections in adult cows with BRD. These results highlight the importance of including these bacteria in the group of etiological agents responsible for the occurrence of BRD in cattle, especially in adult cows with unfavorable immunological conditions, such as recent calving and peak lactation.
引用
收藏
页码:3275 / 3281
页数:7
相关论文
共 32 条
  • [1] Mycoplasma bovis infections in Swiss dairy cattle: a clinical investigation
    Aebi, Marlis
    van den Borne, Bart H. P.
    Raemy, Andreas
    Steiner, Adrian
    Pilo, Paola
    Bodmer, Michele
    [J]. ACTA VETERINARIA SCANDINAVICA, 2015, 57
  • [2] Development of a PCR test for identification of Haemophilus somnus in pure and mixed cultures
    Angen, O
    Ahrens, P
    Tegtmeier, C
    [J]. VETERINARY MICROBIOLOGY, 1998, 63 (01) : 39 - 48
  • [3] Respiratory disease in calves: Microbiological investigations on trans-tracheally aspirated bronchoalveolar fluid and acute phase protein response
    Angen, Oystein
    Thomsen, John
    Larsen, Lars Erik
    Larsen, Jesper
    Kokotovic, Branko
    Heegaard, Peter M. H.
    Enemark, Jorg M. D.
    [J]. VETERINARY MICROBIOLOGY, 2009, 137 (1-2) : 165 - 171
  • [4] RAPID AND SIMPLE METHOD FOR PURIFICATION OF NUCLEIC-ACIDS
    BOOM, R
    SOL, CJA
    SALIMANS, MMM
    JANSEN, CL
    WERTHEIMVANDILLEN, PME
    VANDERNOORDAA, J
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1990, 28 (03) : 495 - 503
  • [5] Effects of storage methods on the recovery of Mycoplasma species from milk samples
    Boonyayatra, S.
    Fox, L. K.
    Besser, T. E.
    Sawant, A.
    Gay, J. M.
    [J]. VETERINARY MICROBIOLOGY, 2010, 144 (1-2) : 210 - 213
  • [6] Gap analysis of Mycoplasma bovis disease, diagnosis and control: An aid to identify future development requirements
    Calcutt, M. J.
    Lysnyansky, I.
    Sachse, K.
    Fox, L. K.
    Nicholas, R. A. J.
    Ayling, R. D.
    [J]. TRANSBOUNDARY AND EMERGING DISEASES, 2018, 65 : 91 - 109
  • [7] Rapid detection and differentiation of bovine herpesvirus 1 and 5 glycoprotein C gene in clinical specimens by multiplex-PCR
    Claus, MP
    Alfieri, AF
    Folgueras-Flatschart, AV
    Wosiacki, SR
    Médici, KC
    Alfieri, AA
    [J]. JOURNAL OF VIROLOGICAL METHODS, 2005, 128 (1-2) : 183 - 188
  • [8] &ITShort communication:&IT Effect of freezer storage time and thawing method on the recovery of &ITMycoplasma bovis&IT from bovine colostrum
    Gille, L.
    Boyen, F.
    Van Driessche, L.
    Valgaeren, B.
    Haesebrouck, F.
    Deprez, P.
    Pardon, B.
    [J]. JOURNAL OF DAIRY SCIENCE, 2018, 101 (01) : 609 - 613
  • [9] Harhay GP, 2017, GENOME ANNOUNCEMENTS, V5, DOI [10.1128/genomeA.01099-17, 10.1128/genomea.01099-17]
  • [10] Molecular survey of infectious agents associated with bovine respiratory disease in a beef cattle feedlot in southern Brazil
    Headley, Selwyn A.
    Okano, Werner
    Balbo, Luciana C.
    Marcasso, Rogerio A.
    Oliveira, Thalita E.
    Alfieri, Alice F.
    Negri Filho, Luiz C.
    Michelazzo, Mariana Z.
    Rodrigues, Silvio C.
    Baptista, Anderson L.
    Saut, Joao Paulo E.
    Alfieri, Amauri A.
    [J]. JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION, 2018, 30 (02) : 249 - 251