Influence of Joule heating during single-cell electroporation simulation under IRE and H-FIRE pulses

被引：2

作者：

Guo, Fei ^{[1
]}

Nie, Xiuwen ^{[1
]}

Hong, Jing ^{[1
]}

Zhang, Yapeng ^{[1
]}

Sun, Jiaguo ^{[1
]}

Zhang, Yu ^{[2
]}

机构：

[1] Chongqing Univ Posts & Telecommun, Inst Ecol Safety, Chongqing 400065, Peoples R China

[2] Chongqing Tradit Chinese Med Hosp, Dept Gynecol & Obstet, Chongqing 400021, Peoples R China

来源：

MATERIALS TODAY COMMUNICATIONS | 2023年 / 36卷

基金：

中国国家自然科学基金;

关键词：

mu sPEFs; Electroporation; Joule heating; Pulse burst repetition frequency (PBRF); BILAYER LIPID-MEMBRANES; IRREVERSIBLE ELECTROPORATION; GENE ELECTROTRANSFER; ELECTRIC BREAKDOWN; REPETITION-RATE; ELECTROCHEMOTHERAPY; NANOSECOND; MUSCLE;

D O I：

10.1016/j.mtcomm.2023.106853

中图分类号：

T [工业技术];

学科分类号：

08 ;

摘要：

Conventional irreversible electroporation (IRE) and high-frequency irreversible electroporation (H-FIRE) pulses have been widely employed in many biomedical and food applications. To investigate the effect of Joule heating generated by pulsed electric fields on cell electroporation, single-cell electroporation models without (EP only) and with the consideration of Joule heating (EP+T) were subjected to the equivalent energy IRE and H-FIRE pulses. The simulation results showed that with the consideration of Joule heating, the transmembrane voltage of the anodic point (TMPA) reached the electroporation threshold (similar to 1 V) faster, and a significant increase in the plasma membrane conductivity (sigma(A)) and pore number (k(p)) was found. We further evaluated the effect of the pulse burst repetition frequency (PBRF) of H-FIRE pulses on cell electroporation based on the EP+T model, and discovered k(p) increased with frequency when PBRF lower than 2 kHz, whereas it decreased with frequency for PBRF higher than 2 kHz. The accumulation of the ratio of the perforation area to the cell membrane area (sum of A(p)/A) during the 400th pulse was also reversed when PBRF was 2 kHz. Our results indicated that Joule heating generated by repetitive pulse delivery would facilitate electroporation, and the effect of PBRF on cell electroporation was non-monotonic.

引用

页数：8