Humulus japonicus ameliorates irritant contact dermatitis by suppressing NF-?B p65-dependent inflammatory responses in mice

被引:2
作者
Kim, Yu-Bin [1 ,2 ]
Kang, Eun-Jung [1 ]
Noh, Jung-Ran [1 ]
An, Jin-Pyo [3 ]
Park, Jong-Tae [2 ]
Oh, Won Keun [3 ]
Kim, Yong-Hoon [1 ,4 ]
Lee, Chul-Ho [1 ,4 ]
机构
[1] Korea Res Inst Biosci & Biotechnol, Lab Anim Resource Ctr, 125 Gwahak, Daejeon 34141, South Korea
[2] Chungnam Natl Univ, Dept Food Sci & Technol, Daejeon 34134, South Korea
[3] Seoul Natl Univ, Res Inst Pharmaceut Sci, Coll Pharm, Korea Bioact Nat Mat Bank, Seoul 08826, South Korea
[4] Korea Univ Sci & Technol, Korea Res Inst Biosci & Biotechnol, Sch Biosci, Dept Funct Genom, Daejeon 34141, South Korea
关键词
Humulus japonicus; irritant contact dermatitis; luteolin; 12-O-tetradecanoylphorbol-13-acetate; NF-kappa B; p65; MECHANISMS; IMMUNITY; BARRIER;
D O I
10.3892/etm.2023.12145
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
As a type of contact dermatitis (CD), irritant CD (ICD) is an acute skin inflammation caused by external irritants, such as soap, water and chemicals. Humulus japonicus (HJ) is a herbal medicine widely distributed in Asian countries and has anti-inflammatory, antimicrobial and antioxidant effects. The current study aimed to investigate the antiderma-titis effect of HJ on ICD and determine the molecular basis of this effect using 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced dermatitis mice models and lipopolysaccharide (LPS) -stimulated RAW264.7 cells. Mice were orally administered HJ and luteolin, the major compound in HJ, and topically administered TPA on the right ear to induce dermatitis. Topical application of TPA induced ear redness, oedema and increased infiltration of neutrophils and macrophages, which ameliorated following HJ and luteolin administration. The gene expression levels of inflammatory cell migrating chemokines, chemokine ligand 3 (CCL3) and chemokine (C-X-C motif) ligand 2 (CXCL2), and pro-inflammatory cytokine, IL-1 beta, were reduced in the ears of HJ- and luteolin-treated mice. HJ and luteolin also inhibited the gene expression of chemokines, CCL3 and CXCL2, and pro-inflammatory cytokines, IL-1 beta, IL-6 and TNF-alpha, in LPS-stimulated RAW264.7 cells. Moreover, HJ and luteolin decreased the expression levels of two key inflammatory enzymes, cyclooxygenase-2 (COX2) and inducible nitric oxide synthase (iNOS), and total and active phosphorylation of NF-kappa B p65. These results suggest that HJ could have a protective effect against ICD by suppressing inflammatory responses; therefore, HJ is a promising therapeutic strategy for ICD treatment.
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页数:11
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