Comparative pathogenicity of single and mixed drug-resistant Trypanosoma brucei brucei and Trypanosoma congolense infections in rats

被引：1

作者：

Obi, Chukwunonso F. ^{[1
]}

Okpala, Michael I. ^{[1
]}

Anyogu, Davinson C. ^{[2
]}

Onyeabo, Amaechi ^{[3
]}

Aneru, Ganiyu E. ^{[1
]}

Ezeh, Ikenna O. ^{[1
]}

Ezeokonkwo, Romanus C. ^{[1
]}

机构：

[1] Univ Nigeria, Fac Vet Med, Dept Vet Parasitol & Entomol, Nsukka, Enugu, Nigeria

[2] Univ Nigeria, Fac Vet Med, Dept Vet Pathol & Microbiol, Nsukka, Enugu, Nigeria

[3] Michael Okpara Univ Agr, Coll Vet Med, Dept Vet Parasitol & Entomol, Umudike, Abia, Nigeria

来源：

RESEARCH IN VETERINARY SCIENCE | 2023年 / 162卷

关键词：

Drug -resistant trypanosomes; Pathogenicity; Clinical findings; Haematology; Rats; CATTLE;

D O I：

10.1016/j.rvsc.2023.104946

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Drug-resistant trypanosomes are widespread in sub-Saharan Africa and in conjunction with the drug-sensitive phenotypes cause a serious endemic wasting disease in animals. We evaluated the pathogenicity of single and mixed drug-resistant Trypanosoma brucei brucei and T. congolense isolates in 35 female rats, randomly divided into seven groups (1-7) of five rats. Group 1 was the uninfected control. Groups 2 and 3 were infected with drugsensitive T. brucei brucei and T. congolense, respectively, whereas groups 4 and 5 were infected with multidrug-resistant T. brucei brucei and T. congolense respectively. Group 6 were infected with drug-sensitive T. brucei brucei and T. congolense while group 7 were infected with multidrug-resistant T. brucei brucei and T. congolense. Parasitaemia kinetics, haematological parameters, body weight, clinical signs, survival time, gross and histopathological changes in the spleen were evaluated. Parasitaemia occurred between day 3-9 postinfection in all the infected groups. Rats in groups 4 and 7 had markedly prolonged (p < 0.05) pre-patent period, days to first peak parasitaemia, survival time, and lower (p < 0.05) parasitaemia level than groups 2 and 6 rats while these parameters were comparable for groups 3 and 5 rats. Anaemia was noted in the infected groups but the severity did not vary amongst the infected groups. Severe clinical signs and splenic lesions were noted in rats infected with drug-sensitive trypanosome species compared to the multidrug-resistant species. Therefore, we conclude that the trypanosome isolates were pathogenic. However, the drug-sensitive T. brucei brucei and mixed drug-sensitive trypanosome infections were more pathogenic than their multidrug-resistant counterparts.

引用

页数：12