Protaetia brevitarsis Extract Attenuates RANKL-Induced Osteoclastogenesis by Inhibiting the JNK/NF-?B/PLC?2 Signaling Pathway

被引：3
作者：
Jang, Hye-Yeon [1 ]
Kim, Jeong-Mi [2 ]
Kim, Jong-Suk [2 ,3 ]
Kim, Byeong-Soo [4 ]
Lee, Young-Rae [5 ]
Bae, Jun Sang [6 ]
机构：
[1] Korea Res Inst Chem Technol KRICT, Infect Dis Therapeut Res Ctr, Daejeon 34114, South Korea
[2] Jeonbuk Natl Univ, Med Sch, Dept Biochem, 20 Geonji Ro, Jeonju 54907, South Korea
[3] Jeonbuk Natl Univ, BK21FOUR Century Med Sci Creat Human Resource Dev, 567 Baekje Daero, Jeonju 54896, South Korea
[4] Kongju Natl Univ, Dept Compan & Lab Anim Sci, Yesan 32439, South Korea
[5] Wonkwang Univ, Inst Biomat Implant, Sch Dent, Dept Oral Biochem, 460, Iksan 54538, South Korea
[6] Wonkwang Univ, Coll Korean Med, Dept Pathol, 460, Iksan 54538, South Korea
来源：
NUTRIENTS | 2023年 / 15卷 / 14期
关键词：
Protaetia brevitarsis; edible insect; osteoclast differentiation; JNK; NF-& kappa; B; PLC & gamma; 2; NF-KAPPA-B; RECEPTOR ACTIVATOR; NUCLEAR-FACTOR; FATTY-ACIDS; BONE-RESORPTION; UP-REGULATION; C-FOS; DIFFERENTIATION; EXPRESSION; LIGAND;
D O I：
10.3390/nu15143193
中图分类号：
R15 [营养卫生、食品卫生]; TS201 [基础科学];
学科分类号：
100403 ;
摘要：
Protaetia brevitarsis (PB)-derived bioactive substances have been used as food and medicine in many Asian countries because of their antioxidant, antidiabetic, anti-cancer, and hepatoprotective properties. However, the effect of PB extracts (PBE) on osteoclast differentiation is unclear. In this study, we investigated the effect of PBE on RANKL-induced osteoclastogenesis in mouse bone marrow-derived macrophages (BMMs). To investigate the cytotoxicity of PBE, the viability of BMMs was confirmed via MTT assay. Tartrate-resistant acid phosphatase (TRAP) staining and pit assays were performed to confirm the inhibitory effect of PBE on osteoclast differentiation and bone resorption. The expression levels of osteoclast differentiation-related genes and proteins were evaluated using quantitative real-time PCR and Western blotting. PBE attenuated osteoclastogenesis in BMMs in TRAP and pit assays without cytotoxicity. The expression levels of osteoclast marker genes and proteins induced by RANKL were decreased after PBE treatment. PBE suppressed osteoclastogenesis by inhibiting the RANKL-induced activated JNK/NF-?B/PLC?2 signaling pathway and the expression of NFATc1 and c-Fos. Collectively, these results suggest that PBE could be a potential therapeutic strategy or functional product for osteoclast-related bone disease.
引用
收藏
页数：16
相关论文
共 50 条
  • [1] Crocin inhibits RANKL-induced osteoclastogenesis by regulating JNK and NF-κB signaling pathways
    Shi, Liping
    Zhao, Suping
    Chen, Qian
    Wu, Youwei
    Zhang, Jian
    Li, Na
    MOLECULAR MEDICINE REPORTS, 2018, 17 (06) : 7947 - 7951
  • [2] Punicalagin inhibits RANKL-induced osteoclastogenesis by suppressing NF-κB and MAPK signaling pathways
    Chu, Genglei
    Zhang, Weidong
    Chen, Mimi
    Yang, Huilin
    Yuan, Zhangqin
    INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE, 2018, 11 (07): : 6571 - 6582
  • [3] Mangiferin Attenuates Osteoclastogenesis, Bone Resorption, and RANKL-Induced Activation of NF-κB and ERK
    Ang, Estabelle
    Liu, Qian
    Qi, Ming
    Liu, Hua G.
    Yang, Xiaohong
    Chen, Honghui
    Zheng, Ming H.
    Xu, Jiake
    JOURNAL OF CELLULAR BIOCHEMISTRY, 2011, 112 (01) : 89 - 97
  • [4] Bergapten suppresses RANKL-induced osteoclastogenesis and ovariectomy-induced osteoporosis via suppression of NF-κB and JNK signaling pathways
    Chen, Guiping
    Xu, Qiang
    Dai, Min
    Liu, Xuqiang
    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2019, 509 (02) : 329 - 334
  • [5] Cryptotanshinone inhibits RANKL-induced osteoclastogenesis by regulating ERK and NF-κB signaling pathways
    Wang, Weizhuo
    Huang, Mengdi
    Hui, Yigeng
    Yuan, Puwei
    Guo, Xiong
    Wang, Kunzheng
    JOURNAL OF CELLULAR BIOCHEMISTRY, 2019, 120 (05) : 7333 - 7340
  • [6] Gentiopicroside inhibits RANKL-induced osteoclastogenesis by regulating NF-κB and JNK signaling pathways
    Chen, Fangqing
    Xie, Lin
    Kang, Ran
    Deng, Rongrong
    Xi, Zhipeng
    Sun, Daoxi
    Zhu, Jin
    Wang, Liming
    BIOMEDICINE & PHARMACOTHERAPY, 2018, 100 : 142 - 146
  • [7] Peiminine Suppresses RANKL-Induced Osteoclastogenesis by Inhibiting the NFATc1, ERK, and NF-κB Signaling Pathways
    Zhu, Mengbo
    Xu, Wenbin
    Jiang, Jiuzhou
    Wang, Yining
    Guo, Yanjing
    Yang, Ruijia
    Chang, Yaqiong
    Zhao, Bin
    Wang, Zhenyu
    Zhang, Jianfeng
    Wang, Te
    Shangguan, Liqin
    Wang, Shaowei
    FRONTIERS IN ENDOCRINOLOGY, 2021, 12
  • [8] Carnosol inhibits osteoclastogenesis in vivo and in vitro by blocking the RANKL-induced NF-?B signaling pathway
    CA, P. A. N., I
    YAN, S. H. I. C. H. A. N. G.
    LU, Y. A. N.
    ZHOU, X. I. A. O. X. I. A. O.
    WANG, X. I. U. H. U. I.
    WANG, M. I. N. G. H. U. I.
    YIN, Z. H. I. F. E. N. G.
    MOLECULAR MEDICINE REPORTS, 2022, 26 (01)
  • [9] Hispidulin attenuates bone resorption and osteoclastogenesis via the RANKL-induced NF-κB and NFATc1 pathways
    Nepal, Manoj
    Choi, Hwa Jung
    Choi, Bo-Yun
    Yang, Moon-Shik
    Chae, Jung-Il
    Li, Liang
    Soh, Yunjo
    EUROPEAN JOURNAL OF PHARMACOLOGY, 2013, 715 (1-3) : 96 - 104
  • [10] Icariin inhibits RANKL-induced osteoclastogenesis via modulation of the NF-κB and MAPK signaling pathways
    Xu, Qiang
    Chen, Guiping
    Liu, Xuqiang
    Dai, Min
    Zhang, Bin
    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2019, 508 (03) : 902 - 906
12345