Analysis of the Differentially Expressed Proteins and Metabolic Pathways of Honeybush (Cyclopia subternata) in Response to Water Deficit Stress

被引：2

作者：

Mahlare, Mary-Jane S. ^{[1
,2
]}

Husselmann, Lizex ^{[3
]}

Lewu, Muinat N. N. ^{[1
]}

Bester, Cecilia ^{[1
]}

Lewu, Francis B. B. ^{[2
]}

Caleb, Oluwafemi James ^{[4
,5
]}

机构：

[1] Agr Res Council ARC Infruitec Nietvoorbij, ZA-7599 Stellenbosch, South Africa

[2] Cape Peninsula Univ Technol, Fac Appl Sci, Dept Agr, Wellington Campus,Private Bag X8, ZA-7654 Wellington, South Africa

[3] Univ Western Cape, Dept Biotechnol, ZA-7535 Bellville, South Africa

[4] Stellenbosch Univ, Africa Inst Postharvest Technol, Fac Agrisci, Private Bag X1, ZA-7602 Matieland, South Africa

[5] Stellenbosch Univ, Fac Agrisci, Dept Hort Sci, ZA-7602 Matieland, South Africa

来源：

PLANTS-BASEL | 2023年 / 12卷 / 11期

基金：

新加坡国家研究基金会;

关键词：

Cyclopia subternata; differentially expressed proteins (DEPs); water deficit stress; carbon fixation; proteomic analysis; TEA; INDUSTRY;

D O I：

10.3390/plants12112181

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Honeybush (Cyclopia spp.) is a rich source of antioxidant properties and phenolic compounds. Water availability plays a crucial role in plant metabolic processes, and it contributes to overall quality. Thus, this study aimed to investigate changes in molecular functions, cellular components, and biological processes of Cyclopia subternata exposed to different water stress conditions, which include well-watered (as Control, T1), semi-water stressed (T2), and water-deprived (T3) potted plants. Samples were also collected from a well-watered commercial farm first cultivated in 2013 (T13) and then cultivated in 2017 (T17) and 2019 (T19). Differentially expressed proteins extracted from C. subternata leaves were identified using LC-MS/MS spectrometry. A total of 11 differentially expressed proteins (DEPs) were identified using Fisher's exact test (p < 0.00100). Only a-glucan phosphorylase was found to be statistically common between T17 and T19 (p < 0.00100). Notably, a-glucan phosphorylase was upregulated in the older vegetation (T17) and downregulated in T19 by 1.41-fold. This result suggests that a-glucan phosphorylase was needed in T17 to support the metabolic pathway. In T19, five DEPs were upregulated, while the other six were downregulated. Based on gene ontology, the DEPs in the stressed plant were associated with cellular and metabolic processes, response to stimulus, binding, catalytic activity, and cellular anatomical entity. Differentially expressed proteins were clustered based on the Kyoto Encyclopedia of Genes and Genomes (KEGG), and sequences were linked to metabolic pathways via enzyme code and KEGG ortholog. Most proteins were involved in photosynthesis, phenylpropanoid biosynthesis, thiamine, and purine metabolism. This study revealed the presence of trans-cinnamate 4-monooxygenase, an intermediate for the biosynthesis of a large number of substances, such as phenylpropanoids and flavonoids.

引用

页数：18