Introduction: Lung cancer has long been at the forefront of all cancers in terms of incidence and mortality. Lung adenocarcinoma is the most common type of lung cancer, accounting for 40% of all lung cancer types. Exosomes can act as biomarkers of tumors and thus play an important role.Methods: In this article, high-throughput sequencing of miRNAs in plasma exosomes from lung adenocarcinoma patients and healthy individuals was performed to obtain 87 upregulated miRNAs, which were then combined with data from the GSE137140 database uploaded by others for screening. The database included 1566 preoperative lung cancer patients, 180 postoperative patients, and 1774 non-cancerous controls. We overlapped the miRNAs upregulated in the serum of lung cancer patients in the database relative to those of non-cancer controls and post-operative patients with the upregulated miRNAs obtained from our next-generation sequencing to obtain nine miRNAs. Two miRNAs that were not reported as tumor markers in lung cancer, hsa-miR-4454 and hsa-miR-619-5p, were selected from them and then validated by qRT-PCR, and further analysis of miRNAs was performed using bioinformatics.Results: Real-time quantitative PCR showed that the expression levels of hsa-miR-4454 and hsa-miR-619-5p in plasma exosomes of patients with lung adenocarcinoma were significantly up-regulated. The AUC values of hsa-miR-619-5p and hsa-miR-4454 were 0.906 and 0.975, respectively, both greater than 0.5, showing good performance. The target genes of miRNAs were screened by bioinformatics methods, and the regulatory network between miRNAs and lncRNAs and mRNAs was studied.Discussion: Our work demonstrated that hsa-miR-4454 and hsa-miR-619-5p have the potential to be used as biomarkers for the early diagnosis of lung adenocarcinoma.
机构:
Howard Hughes Med Inst, Whitehead Inst Biomed Res, Cambridge, MA 02138 USA
MIT, Dept Biol, Cambridge, MA 02139 USA
MIT, Computat & Syst Biol Program, Cambridge, MA 02139 USAHoward Hughes Med Inst, Whitehead Inst Biomed Res, Cambridge, MA 02138 USA
Agarwal, Vikram
;
Bell, George W.
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Whitehead Inst Biomed Res, Bioinformat & Res Comp, Cambridge, MA USAHoward Hughes Med Inst, Whitehead Inst Biomed Res, Cambridge, MA 02138 USA
Bell, George W.
;
Nam, Jin-Wu
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h-index: 0
机构:
Howard Hughes Med Inst, Whitehead Inst Biomed Res, Cambridge, MA 02138 USA
MIT, Dept Biol, Cambridge, MA 02139 USA
Hanyang Univ, Coll Nat Sci, Dept Life Sci, Seoul 133791, South KoreaHoward Hughes Med Inst, Whitehead Inst Biomed Res, Cambridge, MA 02138 USA
Nam, Jin-Wu
;
Bartel, David P.
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机构:
Howard Hughes Med Inst, Whitehead Inst Biomed Res, Cambridge, MA 02138 USA
MIT, Dept Biol, Cambridge, MA 02139 USAHoward Hughes Med Inst, Whitehead Inst Biomed Res, Cambridge, MA 02138 USA
机构:
Ctr Canc Biol, Adelaide, SA 5000, Australia
Univ South Australia, Adelaide, SA 5000, Australia
Univ Adelaide, Dept Med, Adelaide, SA 5005, AustraliaCtr Canc Biol, Adelaide, SA 5000, Australia
Bracken, Cameron P.
;
Scott, Hamish S.
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Ctr Canc Biol, Adelaide, SA 5000, Australia
Univ South Australia, Adelaide, SA 5000, Australia
Univ Adelaide, Dept Med, Adelaide, SA 5005, AustraliaCtr Canc Biol, Adelaide, SA 5000, Australia
Scott, Hamish S.
;
Goodall, Gregory J.
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h-index: 0
机构:
Ctr Canc Biol, Adelaide, SA 5000, Australia
Univ South Australia, Adelaide, SA 5000, Australia
Univ Adelaide, Dept Med, Adelaide, SA 5005, AustraliaCtr Canc Biol, Adelaide, SA 5000, Australia
机构:
Indiana Univ Sch Med, Med Sci Program, Bloomington, IN USAIndiana Univ Sch Med, Med Sci Program, Bloomington, IN USA
Dasari, Subramanyam
;
Pandhiri, Taruni
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Indiana Univ Sch Med, Med Sci Program, Bloomington, IN USAIndiana Univ Sch Med, Med Sci Program, Bloomington, IN USA
Pandhiri, Taruni
;
Grassi, Tommaso
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Mayo Clin, Dept Obstet & Gynecol, Rochester, MN USAIndiana Univ Sch Med, Med Sci Program, Bloomington, IN USA
Grassi, Tommaso
;
Visscher, Daniel W.
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Mayo Clin, Dept Lab Med & Pathol, Rochester, MN USAIndiana Univ Sch Med, Med Sci Program, Bloomington, IN USA
Visscher, Daniel W.
;
Multinu, Francesco
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Mayo Clin, Dept Obstet & Gynecol, Rochester, MN USAIndiana Univ Sch Med, Med Sci Program, Bloomington, IN USA
Multinu, Francesco
;
Agarwal, Komal
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Indiana Univ Sch Med, Med Sci Program, Bloomington, IN USA
St Vincent Dunn Hosp, Dept Obstet & Gynecol, Bedford, IN USAIndiana Univ Sch Med, Med Sci Program, Bloomington, IN USA
Agarwal, Komal
;
Mariani, Andrea
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Mayo Clin, Dept Obstet & Gynecol, Rochester, MN USAIndiana Univ Sch Med, Med Sci Program, Bloomington, IN USA
Mariani, Andrea
;
Shridhar, Viji
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Mayo Clin, Dept Lab Med & Pathol, Rochester, MN USAIndiana Univ Sch Med, Med Sci Program, Bloomington, IN USA
Shridhar, Viji
;
Mitra, Anirban K.
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机构:
Indiana Univ Sch Med, Med Sci Program, Bloomington, IN USA
Indiana Univ Sch Med, Dept Med & Mol Genet, Indianapolis, IN 46202 USA
Indiana Univ, Melvin & Bren Simon Canc Ctr, Indianapolis, IN 46204 USAIndiana Univ Sch Med, Med Sci Program, Bloomington, IN USA
机构:
Howard Hughes Med Inst, Whitehead Inst Biomed Res, Cambridge, MA 02138 USA
MIT, Dept Biol, Cambridge, MA 02139 USA
MIT, Computat & Syst Biol Program, Cambridge, MA 02139 USAHoward Hughes Med Inst, Whitehead Inst Biomed Res, Cambridge, MA 02138 USA
Agarwal, Vikram
;
Bell, George W.
论文数: 0引用数: 0
h-index: 0
机构:
Whitehead Inst Biomed Res, Bioinformat & Res Comp, Cambridge, MA USAHoward Hughes Med Inst, Whitehead Inst Biomed Res, Cambridge, MA 02138 USA
Bell, George W.
;
Nam, Jin-Wu
论文数: 0引用数: 0
h-index: 0
机构:
Howard Hughes Med Inst, Whitehead Inst Biomed Res, Cambridge, MA 02138 USA
MIT, Dept Biol, Cambridge, MA 02139 USA
Hanyang Univ, Coll Nat Sci, Dept Life Sci, Seoul 133791, South KoreaHoward Hughes Med Inst, Whitehead Inst Biomed Res, Cambridge, MA 02138 USA
Nam, Jin-Wu
;
Bartel, David P.
论文数: 0引用数: 0
h-index: 0
机构:
Howard Hughes Med Inst, Whitehead Inst Biomed Res, Cambridge, MA 02138 USA
MIT, Dept Biol, Cambridge, MA 02139 USAHoward Hughes Med Inst, Whitehead Inst Biomed Res, Cambridge, MA 02138 USA
机构:
Ctr Canc Biol, Adelaide, SA 5000, Australia
Univ South Australia, Adelaide, SA 5000, Australia
Univ Adelaide, Dept Med, Adelaide, SA 5005, AustraliaCtr Canc Biol, Adelaide, SA 5000, Australia
Bracken, Cameron P.
;
Scott, Hamish S.
论文数: 0引用数: 0
h-index: 0
机构:
Ctr Canc Biol, Adelaide, SA 5000, Australia
Univ South Australia, Adelaide, SA 5000, Australia
Univ Adelaide, Dept Med, Adelaide, SA 5005, AustraliaCtr Canc Biol, Adelaide, SA 5000, Australia
Scott, Hamish S.
;
Goodall, Gregory J.
论文数: 0引用数: 0
h-index: 0
机构:
Ctr Canc Biol, Adelaide, SA 5000, Australia
Univ South Australia, Adelaide, SA 5000, Australia
Univ Adelaide, Dept Med, Adelaide, SA 5005, AustraliaCtr Canc Biol, Adelaide, SA 5000, Australia
机构:
Indiana Univ Sch Med, Med Sci Program, Bloomington, IN USAIndiana Univ Sch Med, Med Sci Program, Bloomington, IN USA
Dasari, Subramanyam
;
Pandhiri, Taruni
论文数: 0引用数: 0
h-index: 0
机构:
Indiana Univ Sch Med, Med Sci Program, Bloomington, IN USAIndiana Univ Sch Med, Med Sci Program, Bloomington, IN USA
Pandhiri, Taruni
;
Grassi, Tommaso
论文数: 0引用数: 0
h-index: 0
机构:
Mayo Clin, Dept Obstet & Gynecol, Rochester, MN USAIndiana Univ Sch Med, Med Sci Program, Bloomington, IN USA
Grassi, Tommaso
;
Visscher, Daniel W.
论文数: 0引用数: 0
h-index: 0
机构:
Mayo Clin, Dept Lab Med & Pathol, Rochester, MN USAIndiana Univ Sch Med, Med Sci Program, Bloomington, IN USA
Visscher, Daniel W.
;
Multinu, Francesco
论文数: 0引用数: 0
h-index: 0
机构:
Mayo Clin, Dept Obstet & Gynecol, Rochester, MN USAIndiana Univ Sch Med, Med Sci Program, Bloomington, IN USA
Multinu, Francesco
;
Agarwal, Komal
论文数: 0引用数: 0
h-index: 0
机构:
Indiana Univ Sch Med, Med Sci Program, Bloomington, IN USA
St Vincent Dunn Hosp, Dept Obstet & Gynecol, Bedford, IN USAIndiana Univ Sch Med, Med Sci Program, Bloomington, IN USA
Agarwal, Komal
;
Mariani, Andrea
论文数: 0引用数: 0
h-index: 0
机构:
Mayo Clin, Dept Obstet & Gynecol, Rochester, MN USAIndiana Univ Sch Med, Med Sci Program, Bloomington, IN USA
Mariani, Andrea
;
Shridhar, Viji
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h-index: 0
机构:
Mayo Clin, Dept Lab Med & Pathol, Rochester, MN USAIndiana Univ Sch Med, Med Sci Program, Bloomington, IN USA
Shridhar, Viji
;
Mitra, Anirban K.
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h-index: 0
机构:
Indiana Univ Sch Med, Med Sci Program, Bloomington, IN USA
Indiana Univ Sch Med, Dept Med & Mol Genet, Indianapolis, IN 46202 USA
Indiana Univ, Melvin & Bren Simon Canc Ctr, Indianapolis, IN 46204 USAIndiana Univ Sch Med, Med Sci Program, Bloomington, IN USA