Iroquois homeobox 3 regulates odontoblast proliferation and differentiation mediated by Wnt5a expression

被引：2

作者：

Narwidina, Anrizandy ^{[1
,2
]}

Miyazaki, Aya ^{[2
]}

Iwata, Kokoro ^{[3
]}

Kurogoushi, Rika ^{[3
]}

Sugimoto, Asuna ^{[3
]}

Kudo, Yasusei ^{[4
]}

Kawarabayashi, Keita ^{[2
]}

Yamakawa, Yoshihito ^{[1
,2
]}

Akazawa, Yuki ^{[2
]}

Kitamura, Takamasa ^{[2
]}

Nakagawa, Hiroshi ^{[2
]}

Yamaguchi -Ueda, Kimiko ^{[2
]}

Hasegawa, Tomokazu ^{[2
]}

Yoshizaki, Keigo ^{[5
]}

Fukumoto, Satoshi ^{[6
]}

Yamamoto, Akihito ^{[7
]}

Ishimaru, Naozumi ^{[8
]}

Iwasaki, Tomonori ^{[2
]}

Iwamoto, Tsutomu ^{[3
]}

机构：

[1] Tokushima Univ, Grad Sch, Grad Sch Oral Sci, Dept Pediat Dent, Tokushima 7708504, Japan

[2] Tokushima Univ, Inst Biomed Sci, Grad Sch, Dept Pediat Dent, Tokushima 7708504, Japan

[3] Tokyo Med & Dent Univ, Grad Sch Med & Dent Sci, Dept Pediat Dent Special Needs Dent, Div Oral Hlth Sci, Tokyo 1138549, Japan

[4] Tokushima Univ, Inst Biomed Sci, Grad Sch, Dept Oral Biosci, Tokushima 7708504, Japan

[5] Kyushu Univ, Fac Dent Sci, Orthodont & Dentofacial Orthoped Sect, Div Oral Hlth Growth & Dev, Fukuoka 8128582, Japan

[6] Kyushu Univ, Fac Dent Sci, Pediat Dent Sect, Div Oral Hlth Growth & Dev, Fukuoka 8128582, Japan

[7] Tokushima Univ, Inst Biomed Sci, Grad Sch, Dept Tissue Regenerat, Tokushima 7708504, Japan

[8] Tokushima Univ, Inst Biomed Sci, Grad Sch, Dept Oral Mol Pathol, Tokushima 7708504, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2023年 / 650卷

关键词：

Iroquois homeobox genes; Irx3; Odontoblasts; Cell proliferation; Cell differentiation; Wnt5a; GENES; IRX5; ORGANIZATION; MEMBERS; FAMILY;

D O I：

10.1016/j.bbrc.2023.02.004

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Iroquois homeobox (Irx) genes are TALE-class homeobox genes that are evolutionarily conserved across species and have multiple critical cellular functions in fundamental tissue development processes. Previous studies have shown that Irxs genes are expressed during tooth development. However, the precise roles of genes in teeth remain unclear. Here, we demonstrated for the first time that Irx3 is an essential molecule for the proliferation and differentiation of odontoblasts. Using cDNA synthesized from postnatal day 1 (P1) tooth germs, we examined the expression of all Irx genes (Irx1-Irx6) by RT-PCR and found that all genes except Irx4 were expressed in the tooth tissue. Irx1-Irx3 a were expressed in the dental epithelial cell line M3H1 cells, while Irx3 and Irx5 were expressed in the dental mesenchymal cell line mDP cells. Only Irx3 was expressed in both undifferentiated cell lines. Immunostaining also revealed the presence of IRX3 in the dental epithelial cells and mesenchymal condensation. Inhibition of endogenous Irx3 by siRNA blocks the proliferation and differentiation of mDP cells. Wnt3a, Wnt5a, and Bmp4 are factors involved in odontoblast differentiation and were highly expressed in mDP cells by quantitative PCR analysis. Interestingly, the expression of Wnt5a (but not Wnt3a or Bmp4) was sup-pressed by Irx3 siRNA. These results suggest that Irx3 plays an essential role in part through the regu-lation of Wnt5a expression during odontoblast proliferation and differentiation. (c) 2023 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

引用

页码：47 / 54

页数：8

共 42 条

[11] The homeodomain transcription factor lrx5 establishes the mouse cardiac ventricular repolarization gradient [J].