Characterization of Biomineralizing and Plant Growth-Promoting Attributes of Lithobiontic Bacteria

The application of mineral-solubilizing, plant growth-promoting bacteria as inoculants offers a promising alternative to chemical fertilizers. In the present study, lithic bacterial isolates were evaluated for mineral solubilization and plant growth-promoting potential. Among the 57 lithic bacterial isolates associated with different rock samples collected from various locations in Meghalaya, India, nine K-solubilizing isolates, six S-solubilizing isolates, five P- and Si-solubilizing isolates, and three Zn-solubilizing isolates with notable indole-3-acetic acid and siderophore production, and ACC deaminase activity were selected for further study. Based on 16S rRNA gene sequence analysis, isolates were affiliated to nine different genera (Arthrobacter, Acinetobacter, Pseudomonas, Halopseudomonas, Bacillus, Neobacillus, Peribacillus, Pantoea, and Priestia). On performing rice seed germination potentials, Pantoea agglomerans BL26, Priestia megaterium BL9, Bacillus subtilis GP2, Halopseudomonas xinjiangensis BL29, and Pseudomonas sp. BM1 were selected for in vitro pot experiments, being the most potent isolates. Following inoculation, all five isolates were found to significantly enhance growth of rice plants (P < 0.05). The maximum shoot length increased due to P. megaterium BL9, the maximum root length increased due to H. xinjiangensis BL29, and the maximum plant fresh weight increased due to P. megaterium BL9. The findings concluded that these five lithic bacterial isolates have potent plant growth-promoting potential with possible prospection through field trials. To the best of available literature, this is a first report on the characterization of lithic bacterial isolates as mineral solubilizers and plant growth promoters.