The stem cell transcription factor ZFP296 transforms NIH3T3 cells and promotes anchorage-independent growth of cancer cells

被引：1
|
作者：
Mizoue, Yumi [1 ]
Ikeda, Tomomi [1 ]
Ikegami, Takako [1 ]
Riabets, Oleksandra [2 ]
Oishi, Yoshie [3 ]
Tobita, Morikuni [3 ]
Akutsu, Hidenori [4 ]
Hattori, Koichi [5 ]
Heissig, Beate [2 ]
Koide, Hiroshi [1 ]
机构：
[1] Juntendo Univ, Lab Mol & Biochem Res, Biomed Res Core Facil, Grad Sch Med, 2-1-1,Hongo,Bunkyo Ku, Tokyo 1138421, Japan
[2] Juntendo Univ, Dept Res Support Utilizing Bioresource Bank, Grad Sch Med, Tokyo, Japan
[3] Juntendo Univ, Med Technol Innovat Ctr, Res Off Regulatory Sci & Res Eth, Grad Sch Med, Tokyo, Japan
[4] Natl Ctr Child Hlth & Dev, Ctr Regenerat Med, Tokyo, Japan
[5] Juntendo Univ, Ctr Genom & Regenerat Med, Grad Sch Med, Tokyo, Japan
来源：
INTERNATIONAL JOURNAL OF DEVELOPMENTAL BIOLOGY | 2023年 / 67卷 / 04期
关键词：
ZNF296; anchorage-independent growth; oncogene; embryonic stem cells; tumor; EXPRESSION;
D O I：
10.1387/ijdb.230143hk
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Cancer cells and embryonic stem (ES) cells share several biological properties, suggesting that some genes expressed in ES cells may play an important role in cancer cell growth. In this study, we investigated the possible role of zinc finger protein 296 (ZFP296), a transcription factor expressed in ES cells, in cancer development. First, we found that overexpression of Zfp296 in NIH3T3 mouse fibroblasts induced two phenomena indicative of cell transformation: enhanced proliferation under low-serum conditions and anchorage-independent growth. We also found that Zfp296 expression was upregulated in the tumor area of a mouse model of colon carcinogenesis. In addition, the expression levels of ZFP296 in various human cell lines were generally low in normal cells and relatively high in cancer cells. Finally, using a soft agar assay, we found that overexpression of ZFP296 promoted the anchorage-independent growth of cancer cells, while its knockdown had the opposite effect. Overall, these results suggest a possible role of the ES-specific transcription factor ZFP296 in cancer.
引用
收藏
页码：147 / 153
页数：7
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