Direct Detection of Viral Infections from Swab Samples by Probe-Gated Silica Nanoparticle-Based Lateral Flow Assay

被引:0
作者
Durdabak, Dilara Buse [1 ]
Dogan, Soner [2 ]
Tekol, Serap Demir [3 ]
Celik, Caner [4 ]
Ozalp, Veli Cengiz [5 ]
Tuna, Bilge Guvenc [1 ]
机构
[1] Yeditepe Univ, Fac Med, Dept Biophys, TR-34755 Istanbul, Turkiye
[2] Yeditepe Univ, Dept Med Biol, Fac Med, TR-34755 Istanbul, Turkiye
[3] Univ Hlth Sci, Kartal Dr Lutfi Kirdar City Hosp, Dept Clin Microbiol, TR-34865 Istanbul, Turkiye
[4] Mem Sisli Hosp, Dept Emergency Med Serv, Istanbul, Turkiye
[5] Atilim Univ, Fac Med, Dept Med Biol, TR-06830 Ankara, Turkiye
关键词
detection of viral infection; fluorescence-based LFA; gated-SNPs; lateral flow assay; silica nanoparticles; MESOPOROUS SILICA; SYSTEM; DUPLEX;
D O I
10.1002/open.202300120
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Point-of-care diagnosis is crucial to control the spreading of viral infections. Here, universal-modifiable probe-gated silica nanoparticles (SNPs) based lateral flow assay (LFA) is developed in the interest of the rapid and early detection of viral infections. The most superior advantage of the rapid assay is its utility in detecting various sides of the virus directly from the human swab samples and its adaptability to detect various types of viruses. For this purpose, a high concentration of fluorescein and rhodamine B as a reporting material was loaded into SNPs with excellent loading capacity and measured using standard curve, 4.19 mu mol & sdot; g-1 and 1.23 mu mol & sdot; g-1, respectively. As a model organism, severe acute respiratory syndrome coronavirus-2 (CoV-2) infections were selected by targeting its nonstructural (NSP9, NSP12) and envelope (E) genes as target sites of the virus. We showed that NSP12-gated SNPs-based LFA significantly outperformed detection of viral infection in 15 minutes from 0.73 pg & sdot; mL-1 synthetic viral solution and with a dilution of 1 : 103 of unprocessed human samples with an increasing test line intensity compared to steady state (n=12). Compared to the RT-qPCR method, the sensitivity, specificity, and accuracy of NSP12-gated SNPs were calculated as 100 %, 83 %, and 92 %, respectively. Finally, this modifiable nanoparticle system is a high-performance sensing technique that could take advantage of upcoming point-of-care testing markets for viral infection detections. Here, universal-modifiable probe-gated silica nanoparticles (SNPs) based lateral flow assay (LFA) is developed in the interest of the rapid and early detection of viral infections. The most superior advantage of the rapid assay is its utility in detecting various sides of the virus directly from the human swab samples and its adaptability to detect various types of viruses. The NSP12, NSP9, and E gene targets of CoV-2 were used as detection targets.image
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页数:12
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