Effect of Contact Lens Solutions in Stabilizing the Activity of Tear Lysozyme

被引:2
作者
Scheuer, Catherine A. [1 ]
Barniak, Vicki L. [1 ]
Phatak, Nitasha R. [1 ]
Rah, Marjorie J. [1 ]
Reindel, William [1 ]
机构
[1] Bausch & Lomb Inc, Vis Care, Rochester, NY USA
来源
CLINICAL OPTOMETRY | 2023年 / 15卷
关键词
kalifilcon A; silicone hydrogel; homeostasis; protein stabilization; PROTEIN DEPOSITION; DENATURED LYSOZYME; CARE;
D O I
10.2147/OPTO.S404261
中图分类号
R77 [眼科学];
学科分类号
100212 ;
摘要
Purpose: Interactions between tear proteins and the interfaces of contact lenses can be complex and can influence contact lens wear success. Tear proteins, including lysozyme, function to maintain the balance of ocular surface homeostasis, as evidenced by the effects of its conformation relative to stabilizing the tear film and its potential impact on corneal epithelial cells. Contact lens manufacturers include components in lens care and blister package solutions to help stabilize the tear film and preserve homeostasis. This in vitro study was performed to evaluate the ability of daily disposable contact lens package solutions to stabilize lysozyme and preserve its native conformation under denaturing conditions. Methods: Lysozyme was added to contact lens solutions sampled from kalifilcon A, etafilcon A, senofilcon A, narafilcon A, nelfilcon A, verofilcon A, delefilcon A, somofilcon A, and stenfilcon A blister packages, then mixed with the protein denaturant sodium lauryl sulfate. Lysozyme activity was evaluated by adding test solutions to a suspension of Micrococcus luteus. Native lysozyme lyses the Micrococcus luteus cell wall, which decreases suspension turbidity. Stabilization of lysozyme activity was determined by comparing suspension turbidity before and after exposure to test solutions. Results: Lysozyme stabilization was 90.7% for kalifilcon A solution, a statistically significant improvement (p < 0.05) compared to phosphate buffered saline (PBS, negative control). No significant improvement was observed with any other contact lens solution (all lysozyme stabilization < 5.00%). Conclusion: The representative tear protein lysozyme was significantly more stable in the novel kalifilcon A contact lens solution containing multiple moisturizers and osmoprotectants than in PBS or other daily disposable contact lens solutions. The lysozyme activity assay provides mechanistic evidence that the kalifilcon A contact lens solution can stabilize proteins under conditions that typically denature proteins, which may contribute to maintaining ocular surface homeostasis.
引用
收藏
页码:119 / 127
页数:9
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