Development of a Polarization Fluorescent Immunoassay for Sulfathiazole and Its Application for Honey Testing

被引：3

作者：

Nesterenko, I. S. ^{[1
]}

Hendrickson, O. D. ^{[2
]}

Smirnova, N. I. ^{[2
]}

Eremin, S. A. ^{[3
]}

Sotnikov, D. V. ^{[2
]}

机构：

[1] Russian State Ctr Anim Feed & Drug Standardizat &, Fed State Budgetary Inst, Moscow 123022, Russia

[2] Russian Acad Sci, Bach Inst Biochem, Fed Res Ctr, Fundamentals Biotechnol, Moscow 119071, Russia

[3] Moscow MV Lomonosov State Univ, Dept Biol, Moscow 119991, Russia

来源：

APPLIED BIOCHEMISTRY AND MICROBIOLOGY | 2023年 / 59卷 / 01期

基金：

俄罗斯科学基金会;

关键词：

sulfathiazole; antibiotics; fluorescence polarization immunoassay; food quality control; honey; RESIDUES; SULFAMETHAZINE; HEALTH; FOOD;

D O I：

10.1134/S0003683823010052

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A fluorescence polarization immunoassay has been developed for the detection of the antibacterial sulfathiazole. The latter belongs to the sulfonamide class of antibacterial, which is used in beekeeping for the prevention and treatment of infectious diseases of bees. The assay conditions, which ensure the achievement of high analytical characteristics, are determined. The duration of the analysis is 5 min; the limit of sulfathiazole detection is 3 ng/mL. A study of the assay selectivity concerning other sulfonamides demonstrated high specificity only towards sulfathiazole. An optimal method of sample preparation before control honey contamination was proposed. The effectiveness of the developed analysis for the detection of sulfathiazole in honey samples was confirmed. The developed fluorescent polarization immunoassay is a rapid and efficient analytical system for rapid and sensitive control of honey quality and safety.

引用

页码：93 / 98

页数：6

共 50 条

[31] Design, synthesis and characterization of tracers and development of a fluorescence polarization immunoassay for the rapid detection of ractopamine in pork [J].

Dong, Baolei ;

Zhao, Sijun ;

Li, Hongfang ;

Wen, Kai ;

Ke, Yuebin ;

Shen, Jianzhong ;

Zhang, Suxia ;

Shi, Weiming ;

Wang, Zhanhui .

FOOD CHEMISTRY, 2019, 271 :9-17

[32] Development of a lateral flow fluorescent microsphere immunoassay for the determination of sulfamethazine in milk [J].

Rui Chen ;

Heng Li ;

Han Zhang ;

Suxia Zhang ;

Weimin Shi ;

Jianzhong Shen ;

Zhanhui Wang .

Analytical and Bioanalytical Chemistry, 2013, 405 :6783-6789

[33] Single-Domain Nanobodies for Determination of Conformational Changes in Transferrin and Their Use in Fluorescent Polarization Immunoassay [J].

Mukhametova, L. I. ;

Eremin, S. A. ;

Mikhura, I. V. ;

Goryainova, O. S. ;

Sachko, A. M. ;

Ivanova, T. I. ;

Tillib, S. V. .

RUSSIAN JOURNAL OF BIOORGANIC CHEMISTRY, 2025, 51 (02) :702-711

[34] Development of a fluorescence polarization immunoassay for the detection of melamine in milk and milk powder [J].

Wang, Qiang ;

Haughey, Simon A. ;

Sun, Yuan-Ming ;

Eremin, Sergei A. ;

Li, Zhen-Feng ;

Liu, Hui ;

Xu, Zhen-Lin ;

Shen, Yu-Dong ;

Lei, Hong-Tao .

ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2011, 399 (06) :2275-2284

[35] Development of a Homologous Fluorescence Polarization Immunoassay for Diisobutyl Phthalate in Romaine Lettuce [J].

Chen, Yingshan ;

Cui, Xiping ;

Wu, Panpan ;

Jiang, Zhengyun ;

Jiao, Luoying ;

Hu, Qingqing ;

Eremin, Sergei A. ;

Zhao, Suqing .

FOOD ANALYTICAL METHODS, 2017, 10 (02) :449-458

[36] Development of a fluorescence polarization immunoassay for the detection of melamine in milk and milk powder [J].

Qiang Wang ;

Simon A. Haughey ;

Yuan-Ming Sun ;

Sergei A. Eremin ;

Zhen-Feng Li ;

Hui Liu ;

Zhen-Lin Xu ;

Yu-Dong Shen ;

Hong-Tao Lei .

Analytical and Bioanalytical Chemistry, 2011, 399 :2275-2284

[37] Development of a Homologous Fluorescence Polarization Immunoassay for Diisobutyl Phthalate in Romaine Lettuce [J].

Yingshan Chen ;

Xiping Cui ;

Panpan Wu ;

Zhengyun Jiang ;

Luoying Jiao ;

Qingqing Hu ;

Sergei A. Eremin ;

Suqing Zhao .

Food Analytical Methods, 2017, 10 :449-458

[38] Development of a homogeneous immunoassay based on the AlphaLISA method for the detection of chloramphenicol in milk, honey and eggs [J].

Zhang, Yi ;

Huang, Biao ;

Zhang, Jue ;

Wang, Ke ;

Jin, Jian .

JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE, 2012, 92 (09) :1944-1947

[39] Development of Polyclonal Antibody against Clenbuterol for Immunoassay Application [J].

Talib, Nurul Ain A. ;

Salam, Faridah ;

Sulaiman, Yusran .

MOLECULES, 2018, 23 (04)

[40] A reaction based fluorescent sensor for the detection of histamine and its application in fish samples [J].

Gokbel, Fuat ;

Karagoz, Abdurrahman ;

Aydin, Ziya ;

Bayrakci, Mevlut .

SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY, 2025, 343

← 1 2 3 4 5 →