Development of a Polarization Fluorescent Immunoassay for Sulfathiazole and Its Application for Honey Testing

被引：3
作者：
Nesterenko, I. S. [1 ]
Hendrickson, O. D. [2 ]
Smirnova, N. I. [2 ]
Eremin, S. A. [3 ]
Sotnikov, D. V. [2 ]
机构：
[1] Russian State Ctr Anim Feed & Drug Standardizat &, Fed State Budgetary Inst, Moscow 123022, Russia
[2] Russian Acad Sci, Bach Inst Biochem, Fed Res Ctr, Fundamentals Biotechnol, Moscow 119071, Russia
[3] Moscow MV Lomonosov State Univ, Dept Biol, Moscow 119991, Russia
来源：
APPLIED BIOCHEMISTRY AND MICROBIOLOGY | 2023年 / 59卷 / 01期
基金：
俄罗斯科学基金会;
关键词：
sulfathiazole; antibiotics; fluorescence polarization immunoassay; food quality control; honey; RESIDUES; SULFAMETHAZINE; HEALTH; FOOD;
D O I：
10.1134/S0003683823010052
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
A fluorescence polarization immunoassay has been developed for the detection of the antibacterial sulfathiazole. The latter belongs to the sulfonamide class of antibacterial, which is used in beekeeping for the prevention and treatment of infectious diseases of bees. The assay conditions, which ensure the achievement of high analytical characteristics, are determined. The duration of the analysis is 5 min; the limit of sulfathiazole detection is 3 ng/mL. A study of the assay selectivity concerning other sulfonamides demonstrated high specificity only towards sulfathiazole. An optimal method of sample preparation before control honey contamination was proposed. The effectiveness of the developed analysis for the detection of sulfathiazole in honey samples was confirmed. The developed fluorescent polarization immunoassay is a rapid and efficient analytical system for rapid and sensitive control of honey quality and safety.
引用
收藏
页码：93 / 98
页数：6
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