Enzyme-Catalyzed Polymerization of Kraft Lignin from Eucalyptus globulus: Comparison of Bacterial and Fungal Laccases Efficacy

被引:3
作者
Garcia-Fuentevilla, Luisa [1 ]
Dominguez, Gabriela [2 ]
Martin-Sampedro, Raquel [1 ]
Hernandez, Manuel [2 ]
Arias, Maria E. [2 ]
Santos, Jose I. [3 ]
Ibarra, David [1 ]
Eugenio, Maria E. [1 ]
机构
[1] CSIC, Forest Sci Inst ICIFOR INIA, Ctra Coruna Km 7-5, Madrid 28040, Spain
[2] Univ Alcala, Dept Biomed & Biotechnol, Alcala De Henares 28805, Spain
[3] Univ Basque Country UPV EHU, Gen Serv Res SGIKER, Edificio Joxe Mari Korta,Avda Tolosa 72, San Sebastian 20018, Spain
关键词
bacterial laccase; central composite design; characterization; eucalypt; fungal laccase; kraft lignin; polymerization; response surface methodology; LIGNOSULFONATES; DELIGNIFICATION; DETOXIFICATION; ANTIOXIDANT; ALKALINE;
D O I
10.3390/polym15030513
中图分类号
O63 [高分子化学(高聚物)];
学科分类号
070305 ; 080501 ; 081704 ;
摘要
Kraft lignin, a side-stream from the pulp and paper industry, can be modified by laccases for the synthesis of high added-value products. This work aims to study different laccase sources, including a bacterial laccase from Streptomyces ipomoeae (SiLA) and a fungal laccase from Myceliophthora thermophila (MtL), for kraft lignin polymerization. To study the influence of some variables in these processes, a central composite design (CCD) with two continuous variables (enzyme concentration and reaction time) and three levels for each variable was used. The prediction of the behavior of the output variables (phenolic content and molecular weight of lignins) were modelled by means of response surface methodology (RSM). Moreover, characterization of lignins was performed by Fourier-transform infrared (FTIR) spectroscopy and different nuclear magnetic resonance (NMR) spectroscopy techniques. In addition, antioxidant activity was also analyzed. Results showed that lignin polymerization (referring to polymerization as lower phenolic content and higher molecular weight) occurred by the action of both laccases. The enzyme concentration was the most influential variable in the lignin polymerization reaction within the range studied for SiLA laccase, while the most influential variable for MtL laccase was the reaction time. FTIR and NMR characterization analysis corroborated lignin polymerization results obtained from the RSM.
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页数:20
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