Measurement of red blood cell deformability during morphological changes using rotating-glass-plate-based scanning optical tweezers

被引:8
作者
Liu, Rui [1 ]
Shao, Meng [2 ]
Ke, Zeyu [1 ]
Li, Changxu [1 ]
Lu, Fengya [1 ]
Zhong, Min-cheng [2 ]
Mao, Yuxin [1 ]
Wei, Xunbin [1 ,3 ]
Zhong, Zhensheng [1 ]
Zhou, Jinhua [1 ,4 ]
机构
[1] Anhui Med Univ, Sch Biomed Engn, Hefei 230032, Peoples R China
[2] Hefei Univ Technol, Sch Instrument Sci & Optoelect Engn, Hefei 230009, Peoples R China
[3] Peking Univ, Biomed Engn Dept, Beijing 100081, Peoples R China
[4] Anhui Med Univ, Anhui Prov Inst Translat Med, 3D Printing & Tissue Engn Ctr, Hefei 230032, Peoples R China
关键词
MEMBRANE ELASTICITY; STORAGE; MECHANICS; SURVIVAL;
D O I
10.1364/BOE.499018
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
It is important to measure the deformability of red blood cells (RBCs) before transfusion, which is a key factor in the gas transport ability of RBCs and changes during storage of RBCs in vitro. Moreover, the morphology of RBCs also changes during storage. It is proposed that the change in morphology is related to the change in deformability. However, the efficiency of typical methods that use particles as handles is low, especially in the deformability measurement of echinocyte and spherocytes. Therefore, the deformability of RBCs with different morphologies is hard to be measured and compared in the same experiment. In this study, we developed a cost-effective and efficient rotating-glass-plate-based scanning optical tweezers device for the measurement of deformability of RBCs. The performance of this device was evaluated, and the deformability of three types of RBCs was measured using this device. Our results clearly show that the change of erythrocyte morphology from discocyte to echinocyte and spherocyte during storage in vitro is accompanied by a decrease in deformability.
引用
收藏
页码:4979 / 4989
页数:11
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