The experiment was conducted in Saran house to investigate the effect of melatonin foliar spraying at 0, 10, 20 mg l-1 concentrations of grape (Vitis vinifera L.) cv. Summer Royal one year old transplants on vegetative and chemical parameters under two irrigation water quality [Tap water (EC 1.2 ds m(-1)) and Well water (EC 4.5ds m(-1))]. The use of melatonin spraying coefficients with well water irrigation led to significant positive effects on the traits under study, when transplants spraying with melatonin at (20 mg l(-1)) irrigated with well water compared to transplants that were not sprayed and irrigated with the same type of water, as it caused an increase in main stem length (66.200, 52.500 cm), stem diameter (9.360, 8.500 mm), shoots dry weight (13.80, 8.67 gm), total chlorophyll (0.992, 0.516 mg g(-1) FW), while, reducing proline (109.34, 133.45 mg g(-1) DW) and Chloride ions (0.542,0.646%) in leaves compared to the interaction treatment (without melatonin + irrigation with tap water). While, the transplants treated with melatonin and irrigated with tap water achieved similar results for the above mentioned values with superiority of means of well water treatment.