Comparative Analysis of Campylobacter jejuni and C. coli Isolated from Livestock Animals to C. jejuni and C. coli Isolated from Surface Water Using DNA Sequencing and MALDI-TOF

被引:1
|
作者
Denis, Martine [1 ]
Rose, Valerie [1 ]
Nagard, Berengere [1 ]
Thepault, Amandine [1 ]
Lucas, Pierrick [2 ]
Meunier, Meagan [3 ]
Benoit, Fabienne [3 ]
Wilhem, Amandine [4 ]
Gassilloud, Benoit [4 ]
Cauvin, Elodie [3 ]
Rince, Alain [5 ,7 ]
Gourmelon, Michele [6 ]
机构
[1] ANSES, Hyg & Qual Poultry & Pork Prod Unit, Ploufragan Plouzane Niort Lab, French Agcy Food Environm & Occupat Hlth & Safety, F-22440 Ploufragan, France
[2] ANSES, Ploufragan Plouzane Niort Lab, Viral Genet & Biosecur Unit, F-22440 Ploufragan, France
[3] LABEO, Res Dept, Caen St Lo Lab, F-50000 St Lo, France
[4] ANSES, Nancy Lab, PTF Maldi, F-54000 Nancy, France
[5] Caen Normandie Univ, Bacterial Commun & Antiinfect Strategies Reseach U, UNICAEN, CBSA UR4312, F-14000 Caen, France
[6] French Res Inst Exploitat Sea, Dept Oceanog & Ecosyst Dynam, Coastal Environm Dynam & Pelag Ecol Res Unit, ODE DYNECO PELAGOS,IFREMER, F-29280 Plouzane, France
[7] Caen Normandie Univ, UNICAEN, BIOTARGEN, BioTARGen Lab, F-14280 St Contest, France
来源
PATHOGENS | 2023年 / 12卷 / 09期
关键词
Campylobacter; livestock animals; surface water; MLST; cgMLST; structure; MALDI-TOF; RISK-FACTORS; ANTIBIOTIC-RESISTANCE; POPULATION-STRUCTURE; GENETIC DIVERSITY; PREVALENCE; SPP; IDENTIFICATION; CONTAMINATION; GENOTYPES; EPIDEMIOLOGY;
D O I
10.3390/pathogens12091069
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
This study evaluated the contribution of cattle, sheep, poultry and pigs to the contamination of surface water from rivers by Campylobacter jejuni and C. coli using MLST, cgMLST and considered MALDI-TOF MS as an alternative technique. The 263 strains isolated from cattle (n = 61), sheep (n = 42), poultry (n = 65), pigs (n = 60) and surface water (n = 35) were distributed across 115 sequence types (STs), 49 for C. jejuni and 66 for C. coli. Considering MLST data, 14.2%, 11.4% and 2.8% of the surface water strains could be attributed to cattle, poultry and sheep, respectively, none to pigs, and 85.7% were non-attributed. Analysis of cg-MLST data with STRUCTURE indicated that C. jejuni strains from water were predominantly attributed to poultry (93.5%), weakly to sheep (<1%) and 6.3% non-attributed, and that conversely, C. coli strains from water were predominantly non-attributed (94.3%) and 5.7% attributed to poultry. Considering the protein profiles with a threshold of 94% and 97% of similarity, respectively, strains from surface water could be attributed to poultry (31.4% and 17.1%), and to cattle (17.1% and 5.7%); 54.1% and 77.1% were non-attributed. This study confirmed these livestock animals might contribute to the contamination of surface water, with a level of contribution depending on the typing technique and the method of analysis. MALDI-TOF could potentially be an alternative approach for source attribution.
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页数:16
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