Compact and robust dual-color linearly polarized illumination source for three-photon °uorescence imaging

被引：1

作者：

Song, Jiazheng ^{[1
]}

Zhang, Yanyan ^{[1
]}

Liu, Yuanshan ^{[1
]}

机构：

[1] Northwestern Polytech Univ, Sch Artificial Intelligence Opt & Elect iOPEN, Xian 710072, Shaanxi, Peoples R China

来源：

JOURNAL OF INNOVATIVE OPTICAL HEALTH SCIENCES | 2023年 / 16卷 / 05期

关键词：

Three-photon fluorescence imaging; illumination source; dual-wavelength femtosecond pulse; cascaded Raman effect; graded-index multimode fiber; FREQUENCY-SHIFTED SOLITONS; FIBER LASER; AMPLIFICATION;

D O I：

10.1142/S179354582241005X

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

The miniaturized femtosecond laser in near infrared-II region is the core equipment of three-photon microscopy. In this paper, we design a compact and robust illumination source that emits dual-color linearly polarized light for three-photon microscopy. Based on an all-polarization-maintaining passive mode-locked fiber laser, we shift the center wavelength of the pulses to the 1.7 mu m band utilizing cascade Raman effect, thereby generate dual-wavelength pulses. To enhance clarity, the two wavelengths are separated through the graded-index multimode fiber. Then we obtain the dual-pulse sequences with 1639.4 nm and 1683.7 nm wavelengths, 920 fs pulse duration, and 23.75 MHz pulse repetition rate. The average power of the signal is 53.64mW, corresponding to a single pulse energy of 2.25 nJ. This illumination source can be further amplified and compressed for three-photon fluorescence imaging, especially dual-color three-photon fluorescence imaging, making it an ideal option for biomedical applications.

引用

页数：8