Advancing full-field metrology: rapid 3D imaging with geometric phase ferroelectric liquid crystal technology in full-field optical coherence microscopy

被引:1
|
作者
Zheng, Wei [1 ]
Kou, Shan S. [2 ]
Sheppard, Colin J. R. [3 ,4 ]
Roy, Maitreyee [5 ]
机构
[1] Natl Univ Singapore, Dept Biomed Engn, Singapore 117576, Singapore
[2] La Trobe Univ, Chem & Phys, Bundoora, Vic 3083, Australia
[3] Ist Italiano Tecnol, Nanoscopy & NIC IIT, Via Enrico Melen,83 Edificio B, I-16152 Genoa, Italy
[4] Univ Wollongong, Sch Chem & Mol Biosci, Mol Horizons, Wollongong, NSW 2522, Australia
[5] Univ New South Wales, Sch Optometry & Vis Sci, Sydney, NSW 2052, Australia
关键词
CONFOCAL INTERFERENCE MICROSCOPE; WHITE-LIGHT INTERFEROMETRY; DOMAIN REFLECTOMETRY; SCANNING MICROSCOPY; AXIAL RESOLUTION; SCATTERING; TOMOGRAPHY; HETERODYNE; REFLECTION; RECONSTRUCTION;
D O I
10.1364/BOE.488806
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Optical coherence microscopy (OCM) is a variant of OCT in which a high-numerical aperture lens is used. Full-field OCM (FF-OCM) is an emerging non-invasive, label-free, interferometric technique for imaging of surface structures or semi-transparent biomedical subjects with micron-scale resolutions. Different approaches to three dimensional full-field optical metrology are reviewed. The usual method for the phase-shifting technique in FF-OCM involves mechanically moving a mirror to change the optical path difference for obtaining en-face OCM images. However, with the use of a broadband source in FF-OCM, the phase shifts of different spectral components are not the same, resulting in the ambiguities in 3D image reconstruction. In this study, we demonstrate, by imaging tissues and cells, a unique geometric phase-shifter based on ferroelectric liquid crystal technology, to realize achromatic phase-shifting for rapid three-dimensional imaging in a FF-OCM system.
引用
收藏
页码:3433 / 3445
页数:13
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