Antifungal activities of Bacillus velezensis FJAT-52631 and its lipopeptides against anthracnose pathogen Colletotrichum acutatum

被引:13
作者
Deng, Ying-Jie [1 ,2 ]
Chen, Zheng [1 ]
Ruan, Chuan-Qing [1 ]
Xiao, Rong-Feng [1 ]
Lian, Heng-Ping [3 ]
Liu, Bo [1 ]
Chen, Mei-Chun [1 ,4 ]
Wang, Jie-Ping [1 ,4 ]
机构
[1] Fujian Acad Agr Sci, Agr Bioresources Res Inst, Fuzhou, Peoples R China
[2] Technol Huazhong Agr Univ, Coll Life Sci, Wuhan, Peoples R China
[3] Shunchang Lehuo Laibu Tourism Dev Co Ltd, Nanping, Peoples R China
[4] Fujian Acad Agr Sci, Agr Bioresources Res Inst, Fuzhou 350003, Peoples R China
基金
中国国家自然科学基金;
关键词
antifungal activity; biocontrol; Colletotrichum acutatum; lipopeptides; loquat; BIOLOGICAL-CONTROL; BIOCONTROL AGENT; BACILLOMYCIN D; SUBTILIS FMBJ; FENGYCIN; ROT; FRUIT; IDENTIFICATION; EFFICACY; STRAIN;
D O I
10.1002/jobm.202200489
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
This study was aim at investigating antifungal activities of Bacillus velezensis FJAT-52631 and its lipopeptides against Colletotrichum acutatum ex situ and in situ. The results showed that the strain FJAT-52631 and its crude lipopeptides (10 mg/ml) exhibited strong inhibitory effects on growth of C. acutatum FJAT-30256 with an inhibition rate of 75.3% and an inhibition zone diameter of 17.66 mm, respectively. Both the viable bacterial cultures and lipopeptides of FJAT-52631 could delay the onset of loquat anthracnose by 1 day and lower the incidence of loquat anthracnose in situ. The whole cultures of B. velezensis FJAT-52631 displayed a 50% biocontrol efficacy on loquat anthracnose at the fourth day after inoculation, but the crude lipopeptides not. The average lesion diameter of the whole-culture treated group was 5.62 mm, which was smaller than that of control group (6.81 mm). All the three types of lipopeptides including iturin A, fengycin, and surfactin A secreted from the strain FJAT-52631 exhibited antifungal activities. Among them, surfactin A displayed higher antifungal activity at a concentration of 1.25 mg/mL than other two lipopeptides even if at a concentration of 60 mg/mL. Thus, the results indicated that surfactin A produced by FJAT-52631 played a major role in the biocontrol of the loquat anthracnose. Scanning electron microscopy (SEM) observation revealed the structural deformities in the mycelia of C. acutatum. The above results suggested that the antifungal lipopeptides from B. velezensis FJAT-52631 would be potential in biocontrol against anthracnose disease of loquat caused by C. acutatum.
引用
收藏
页码:594 / 603
页数:10
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